1. Initiation of Replication *Strands must separate DnaA Helicases (DnaB and DnaC) SSBPs (Single Stranded Binding Proteins) DNA gyrase (topoisomerase)

2. *Primase Initiation of Replication

3. The sliding clamp

4. The two strands of a double helix are antiparallel Continuous DNA synthesis = leading strand Discontinuous DNA synthesis = lagging strand

5. DNA Synthesis

8. Completing the process

9. DNA Synthesis Eukaryotes DNA unwinds at a replication origin Two replication forks form Bidirectional synthesis of DNA Much more DNA; complexed with proteins Eukaryotes have much more DNA Yeast 250-400 replicons Mammalian cells up to 25,000 Solution: Multiple replication origins Yeast 4X that of E. coli Drosophila 100X that of E. coli