1. Large Molecule ~ Small Molecule Interactions Projects By the Woonsocket Team: Toni DiMarzio Andrea Harnois Alyshia Johnson Claire Laquerre

2. Triphenyl Propyl Phosphonium Bromide Physical Properties  Appearance: white to off-white crystal  Weight 464.19amu (385.28g/mol)  Melting point: 235-239°C Hazards  Irritant to eyes, respiratory system, and skin.

3. Native Genomic DNA Melting According to the graphs, the native genomic DNA had a melting point of 55°C.

4. Treated Genomic DNA Melting According to the graphs, through our experimental difficulties, the melting point of the treated genomic DNA was 70°C.

5. Electrophoresis First gel with compound concentration of 5µl Second gel with compound concentrations of 2µl and 8µl

6. HyperChem Predictions Major Groove Energy = -917.494 kcal/mol

7. HyperChem Predictions Minor Groove Energy = -917.228 kcal/mol

8. HyperChem Predictions Intercalated Energy Before Optimization = 19850224519.55 kcal/mol Energy After Optimization = - 906.903 kcal/mol

9. HyperChem Predictions Energy = 1517.968 kcal/mol

10. HyperChem Predictions Energy = 1529.708 kcal/mol

11. HyperChem Predictions Energy After Optimization = -311.449

12. In Conclusion… According to our electrophoresis gel experiment, our compound of interest did not bind to the DNA. According to our DNA melting, our compound of interest shifted the melting point of the genomic DNA. According to our HyperChem predictions, our compound of interest has a very stable interaction with DNA.