1. Eukaryotic Linear DNA Synthesis: Mechanism Of Telomere Addition
Note: The 3’ end of the chromosome will always be longer, but now all sequences in the chromosome will be replicated
Courtesy: Alberts et. al. Molecular Biology of the Cell

2. Prokaryotic DNA Replication: Rolling Circle Replication
Rolling circle replication is used to replicate some plasmids
Plasmids are small circular DNA molecules that are double stranded
For rolling circle replication, the outer strand is considered the (+) strand and the inner strand is the (-) strand
To start rolling circle replication, the (+) strand is nicked (cut)
In rolling circle replication, the (+) strand serves as a template for the (-) strand and the (-) strand serves as a template to make the (+) strand

3. Prokaryotic DNA Replication:Rolling Circle Replication
The nick in the (+) strand will create a free 3’ OH and allow for synthesis of a new (+) strand
Synthesis of a new (+) strand will start at the free 3’OH using the (-) strand as a template
Synthesis of a new (+) strand is continuous lending the name rolling circle because we go around the circle
Synthesis of a new (-) strand uses the (+) strand as a template
Synthesis of a new (-) strand occurs in a discontinuous replication

4. Prokaryotic DNA Replication: Evidence For Bi-Directional Replication In Bacteria
Although there are two replication forks created, there are two possible ways that the circular Bacterial chromosome could be replicated
Uni-directional (DNA is replicated at only one of the forks)
Bi-Directional (DNA is replicated at both forks)
Elizabeth Gyurasits and R.B. Wake showed that DNA replication in B. subtilis is bi-directional
Grew B. subtilis in the presence of 3H-Thymidine (weak radioactivity) for a short time
Then switch the B. subtilis to media with a more strongly radiolabeled nucleotide (32P)
Exposed the replicating DNA to autoradiography
Both forks picked up the strongly radiolabeled nucleotide, and must have been replicating at the point where the shift occured