1. A DIELECTROPHORETIC CELL/PARTICLE SEPARATOR FABRICATED BY SPIRAL CHANNELS AND CONCENTRIC GOLD ELECTRODES Reporter : Meng-Hua Chung Professor : 劉承賢教授

2. Outlines Introduction Design principle Simulation Fabrication Result Conclusion

3. Introduction Separation method: Passive : base on geometries Advantages: implementation easy Disadvantages: performance is not very well Active: require actuation mechanism Magnetic force Hydrodynamic force Electrical force

4. Introduction Magnetic force Most materials cannot be magnetized Hydrodynamic force Difficulty in controlling and may damage viable cell Electrical force Electrophoresis Dielectrophoresis(DEP)

5. Introduction Electrophoresis

6. Introduction Dielectrophoresis

7. Design Principle DEP force is formed by non-uniform electric field and non-zero CM factor

8. Design principle Crossover frequency

9. Design pattern

10. simulation

11. Fabrication

12. Result

13. Conclusion This spiral channels have two way to use: Different size particles(R) Different type cells(CM factor) This paper already test different size particles, but the result do not fit the formular.