1. SHEA HIGH SCHOOL July 13-17, 2009 Ms. La Roche Cesar Luzon, Janice Miranda, Maldine Santos, Estefania Tabares.

2. Formula : CH 3 P(C 6 H 5 ) 4 Mol WT : 419.30 Synonyms : Bromure de tétraphénylphosphonium; Bromuro de tetrafenilfosfonio; Classification : ORGANOPHOSPHINES. ORGANOPHOSPHINES Physical state : white to off-white crystalline powder. Melting Point :295 - 299 C Solubility in water : Soluble. Stability: Stable under ordinary conditions.

3. Well 1: 10.0 uL Linearized Plasmid DNA. Well 2: 10.0 uL uncut Plasmid DNA Well 3: 10.0uL linearized plasmid DNA w B-A. Well 4: 10.0 uL uncut plasmid DNA w B-A. Well 5: 5.0 uL Kb ladder 2217211519 2423 27 30 34 41 45

5. Electrophoresis 7-16-09 Distance (mm)Base parirs 1923,130 239,416 276,557 304,361 342,322 412,027 45564 125 DistanceBase pairs DistanceBase pairs E-linearEB-linear plasmid& TPP 22150002117000 Observations : both the linear and the uncut plasmid with TPP travelled 1mm further showing the small molecule bonded with the DNA molecule. X-uncutXB-uncut plasmid& TPP 17380001510000 2490002311000

6. Temp.CºAbsorbance∆T∆ATave∆A/∆T DNA 250.7259 350.7259100300 450.7276100.0017400.00017 550.725410-0.002250-0.00022 600.90850.182657.50.03652 650.944150.036162.50.00722 700.946450.002367.50.00046 750.953150.006772.50.00134 800.984350.031277.50.00624 851.018550.034282.50.00684 901.025550.00787.50.0014 951.034650.009192.50.00182 Temp. CºAbsorbance∆TA∆Tave∆A/∆T DNA+orbizine 250.91 350.91100300 451.04100.13400.013 551.2268100.1868500.01868 601.237750.010957.50.00218 651.25650.018362.50.00366 701.24525-0.010867.5-0.00216 751.283350.038172.50.00762 801.24985-0.033577.5-0.0067 851.306650.056882.50.01136 901.33450.027487.50.00548 951.364250.030292.50.00604

8. Salmon DNA +TPP

9. MAJOR GROOVEMINOR GROOVEINTERCALATING

10. TESTORIENTATIONMAJOR GROVE MINOR GROVE INTERCALATIN G DNA and TPP X-582.33-582.68-574.78 DNA and TPP Y-1203.054-611.55-572.25 DNA and TPP Z-582.55-574.72-572.89

11. Temp. C°Absorbance (nm)Column1Column2Column3Column4 DNAΔTΔTΔAΔATaveΔA/ΔT 500.70650.0152.50.002 550.71650.00157.50.0002 600.71750.07462.50.0148 650.79150.12867.50.0256 700.91950.121750.0242 801.04100.00882.50.0008 851.04850.54287.5 901.595-1.5945 Temp.C°Absorbance (nm)Column1Column2Column3Column4 DNA+TPP ΔTΔAΔATaveΔA/ΔT 501.24950.17852.50.0356 551.42750.18557.50.037 601.61250.02862.50.0056 651.6450.06467.50.0128 701.70450.04272.50.0084 802.017100.27782.50.0554 852.29450.09587.50.019 902.38950.009910.0018 Absorbance at 260 nm

14. Hyperchem analyses led to the prediction that tetraphenylphosphonium Bromide would bond to DNA. The experimental data shows a shift in the melting curve when TPP is added, agreeing with this prediction No significant changes were made in the experimental procedures. Light banding in electrophoresis gels suggest using a larger amount of the Samples. Practice pipetting into wells is suggested. Repeated trials of both experimental procedures are recommended for further research. The melting point of Salmon DNA was 70 ⁰ C on the Smart Spec and 59 ⁰ C on the Cary spectrophotometer. The difference in the melting point could be due to the fluctuating temperatures with the Smart Spec Plus. The melting point of the Salmon DNA with the small molecule TPP: Cary - appears to be 49 ⁰ C. There are three other indications of melting in different phases. Smart Spec – points to two melting points, 68 ⁰ C and 83 ⁰ C.