1. The Protestant Legend Presented by: Thao Cao Mentor: Dr. Hiro Nonogaki Seed Biology Laboratory The Protestant Legend http://www.ccmb.res.in/staff/imran/Resources.html

2. Food Genetic information Dispersal unit http://ucsdnews.ucsd.edu/graphics/images/pod%5B1%5D.gif http://commons.wikimedia.org/wiki/File:Pumpkin_seeds.jpg

3. http://www.haverford.edu/biology/Johnson/Modelingt heOrganism/Modeling_the_Organism.htm http://www.worldofstock.com/closeups/PFO3982.php http://www.gettyimages.com/detail/sb10065002w-001/Digital-Vision Broccoli Cauliflower Brussels Sprouts Brassicaceae Canola

4. Dry seed NF-YA9 http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi NF-YA3 Dry seed High Low Expression

5. Down-regulation of NF-YA gene by microRNA is essential to maintain normal developmental program of seeds.

6. MicroRNA RISC

7. Wild type Control Mutant

8. Conditional expression Chemical Alcohol Heat Shock Chemical Induction

9. Gene of Interest Promoter 5XUAS Switch CsMV Promoter Chemical Induction (ligand: methoxyfenozide) Courtesy of Dr. Roger Beachy, Donald Danforth Plant Science Center VP16 ActivationGAL4 DNA BindingEcdysone Receptor Active

10. Promoter GUS Promoter GUS Uninduced Induced

11. Wild type AGE:GUS - + - + 5 days after Imbibition

12. Uninduced Induced

13. -- ++ NF-YA3 mNF-YA3

14. Actin - + NF-YA9 Wild type NF-YA9 mNF-YA9 -- ++

16. Howard Hughes Medical Institute Dr. Kevin Ahern Dr. Hiro Nonogaki Louis Stokes Alliance for Minority Participation Natalya Goloviznina Theresa Nguyen Dr. Cristina Martinez-Andujar Mariko Nonogaki Satoru Yamagishi