1. I NVESTIGATION OF D YNEIN ’ S L INKER R EGIONS Mentor: Dr. Elisar Barbar Jonathan Yih

2. R ELEVANCE Role in basic life processes http://hopes.stanford.edu/basics/dna/f_b14mit_meta.jpg Insight for dynein- associated problems http://www.thehealthsuccesssite.com/images/muscle-als.jpg

3. G OAL Improve understanding of dynein’s structure http://www.physics.berkeley.edu/research/yildiz/DyneinNew.jpg

4. B ACKGROUND Motor protein Energy Work Functions: Transport Orienting & positioning organelles Cell division http://maepro.uta.edu/loco/pictures/kinesin11.jpg

5. B ACKGROUND Dynein’s structure Heavy Heads & Chains ATP hydrolysis & movement Light & Intermediate Chains Binding IC LC8 Cargo Heavy Heads and Chains Microtubule

6. H YPOTHESIS LC8 binding to IC initiates the assembly of the dynein complex.

7. O VERVIEW Surface Plasmon Resonance Study protein kinetics LC8 with: IC nNOS BIM Swa LC8/IC compex image © Barbar 2007

8. S URFACE P LASMON R ESONANCE Gold Surface Results! Immobilized Protein (LC8) Incident Light Reflected Light Analyte

9. S URFACE P LASMON R ESONANCE http://www.discoversensiq.com/uploads/file/support/spr/Overview_of_SPR.pdf Protein complex formation A + B AB kaka kdkd

10. S URFACE P LASMON R ESONANCE Immobilization 1 2 3 Time (s)

11. SPR: LC8 & BIM OR N NOS

12. SPR: LC8 & IC Res SD 6.704

13. SPR: E XPERIMENTAL D ESIGN Replications Multiple concentrations (5+) Adequate regeneration Reduce mass transport limitations Achieve steady state

14. A CKNOWLEDGEMENTS Special thanks to: Dr. Elisar Barbar The Barbar Lab Group Afua Nyarko,Yujuan Song, &Jessica Morgan Nathan Gillock & Aaron Martin Dr. Kevin Ahern The Howard Hughes Medical Institute