1. Daniel Sayle, Patrick Byrne, Sarah Kemp, Anne Mcleland, Katie Thompson 10/5/2010 TEAM MUNCHIES CRITICAL DESIGN REVIEW Fall 2010 Rev B

2. MISSION OVERVIEW - The purpose of the balloon satellite Astraios is to test whether or not red algae can survive the harsh environment of near space at an altitude of approximately 30,500 meters or 100,000 feet. - Possibility that our research can be applied to other extreme environments such as high mountains, the polar regions of the Earth, and the environment in which the first extra terrestrial settlements will be created when the terra-forming of Mars takes place.

3. OVERVIEW, CTD. The Results of this Mission, depending on whether the samples survive the flight, can provide plausible doubt to the possibility of marine life forms in high pressure environments or even extraterrestrial ones. Due to algal blooms supplying the majority of breathable Oxygen to life under water, the survivability of our samples will provide insight to the possibility of oxygen breathing marine life (unicellular or not) in other atmospheres or hostile environments.

4. HYPOTHESIS Due to the rarity of ice specialized algae, our group is using a very common strain of red algae for the flight. This algae is relatively self-sustaining, and able to live without high amounts of sunlight or warm temperatures. Therefore, our samples are most likely to survive in a standard temperature and pressure environment. Once pressure and temperature begin to decrease as elevation increases, we expect the chances of survival of the algae will decrease exponentially, but are not made impossible. The most likely sample to survive the flight will be the sealed and heated one. However, there is reason to speculate the samples prone to pressure changes may still be able to complete photosynthesis functions.

5. REQUIREMENTS FLOW DOWN LevelRequirementDetail 0 Mission Objectives A Astraios shall reach an altitude of approximately 30,500 m on launch day, November 6, 2010, weighing no more than 850g, and costing less than $300 B Astraios will carry 4 algae samples in order to study the effects of a near space environment upon samples under varying conditions C The algae samples will be successfully recovered and then analyzed DThe results will allow Team Munchies to draw conclusions about algae’s usefulness in a near space environment.

6. CONTINUED 1 A.1 Astraios shall be fully assembled and tested by November 3, 2010 and then turned in to Chris Koehler on November 5, 2010 ready to fly. A.2 Astraios will we weighed following assembly to ensure it is within the weight limit. Reconfiguration will occur if necessary A.3 Budget Manager Sarah Kemp will ensure that the monetary budget is adhered to B.1 Team Munchies will obtain algae from Gulf Coast Ecosystems B.2 Astraios’s design will quarantine each algae sample and allow for open air exposure for the cold samples B.3 The samples will be prepared prior to launch, two in sealed bags and two in vented bags C.1Extensive testing will occur to ensure that the design adequately quarantines and protects the samples

7. CONTINUED C.2 Experiment testing will take place before launch to provide control samples with which to compare the resulting flight samples C.3 Ground samples will be present on launch day for comparison with the pressurized flight samples and redundancy samples will be on board. C.4 The recovered flight samples will be examined visually in a microscope to determine condition D.1 The condition of the flight samples will be compared with the controls established during testing and from ground samples D.2 Temperature data and pressure data will be used to understand under what conditions and at which approximate altitude the samples died if they do die.

8. DESIGN Science Algae Samples- 2 Cold, 2 Warm, 2 Sealed, 2 Not Sealed (Being ordered today…should take a week to arrive) Sealed bags are pressurized at sea level, thus testing whether pressure or temperature will kill the algae sample The samples in the cold will be in cube offshoot(s) on top of main cube There will also be an additional 4 redundancy samples on board Ground samples will be tested at STP simultaneously for control group Two HOBOs (in possession)- 1 for temperature and humidity, and 1 for pressure

9. MISSION REQUIREMENTS Extensive testing will be done to ensure that the satellite can survive flight and landing and the flight string interface will be constructed with plastic tubing, washers, and paperclips. The heater and insulation will be utilized to maintain internal temperature. Prior to turn in, the team will weigh the satellite and modify design if it exceeds 850 grams. Ascent and descent rates will be determined using the ultimate altitude reached by the balloon satellite and the duration of ascent and descent as determined by temperature and pressure data. All required hardware will be accommodated into the design. The outside of the satellite will bear contact information and a US flag. Units will be converted to metric. Transportation to launch has been planned. The team will follow its outlined safety procedures and return all hardware in working condition. The budget manager will be diligent when keeping track of expenses and receipts and hardware will be ordered with Chris Koehler’s CU MasterCard.

10. MASS AND MONEY BUDGET ItemPlace of PurchaseCostWeight Algae Gulf Coast Ecosystems$65120g Camera Provided$0220g Heater Provided$0100g HOBO Provided$030g Pressure Sensor (HOBO) Space Grant$020g Additional Temp. Probe Space Grant$0~12g Battery for HOBO Allied Electronics$10.2410g Plastic Bags Target$5(4)-~5g Plastic Tubing Provided$0~12g Washers Provided$0~50g Paperclips Provided$0~2g Test String Provided$0N/A Foam core Provided$0~50g Hot Glue and Glue Provided$0~20g Aluminum Tape Provided$0~20g Insulation Provided$0~50g Batteries (6-9V, 2-AA) Target$20(3-9V)-108g Dry Ice Safeway$20N/A Styrofoam Cooler Owned$0N/A Weather Station Owned$0N/A Total: $120.24Total: 839g

11. DateEvent September 7, 2010 (T) Request for Proposals September 16, 2010 (Th) Conceptual Design Review (proposal/present) September 23, 2010 (T) [appt.] Authority to Proceed/Order Hardware September 29, 2010 (W) Team Meeting October 4, 2010 (M) Team Meeting October 5, 2010 (T) DD Rev A/B & CDR slides due October 5, 2010 (T) Order algae and battery October 6, 2010 (W) Team Meeting- Construction October 10, 2010 (Sun) Team Meeting- Construction/Structure Testing October 12, 2010 (T) HW 5 due October 13, 2010 (W) Team Meeting- Construction/Structure Testing October 17, 2010 (Sun) Team Meeting- Testing: Payload and Function October 20, 2010 (W) Team Meeting- Testing: Function and Cooler October 24, 2010 (Sun) Team Meeting- Testing: Payload and Revision October 25, 2010 (M) Finish Testing October 26, 2010 (T) Pre-Launch Inspection with Hardware (exterior testing must be complete) October 27, 2010 (W) Loose Ends/ DD Rev C & Slides October 28, 2010 (Th) Mission Simulation (All testing complete) October 31, 2010 (Sun) Loose Ends/ DD Rev C & Slides November 2, 2010 (T) DD Rev C & LRR due (present) November 3, 2010 (W) Finalize Balloon Saat November 5, 2010 (F) [appt.] Final weigh in and TURN IN November 6, 2010 (Sat) LAUNCH November 9, 2010 (T) Have Raw Data November 10, 2010 (W) Team Meeting-Presentation November 14, 2010 (Sun) Team Meeting-Presentation November 17, 2010 (W) Team Meeting-Presentation November 28, 2010 (Sun) Team Meeting-Presentation November 30, 2010 (T) Final Presentation and Report due December 4, 2010 (Sat) Design Expo & DD Rev D due December 7, 2010 (T) Hardware TURN IN

12. FUNCTIONAL DESIGN DOC.

13. 3D MODELS

15. TEST PLAN No testing has begun yet Payload testing- Whip Test, Drop Test, Cooler Test, Subsystems Test, Camera Test Algae Testing- Placing it in a cooler of dry ice to test how it will survive Deliberately kill sample of algae to hypothesize what its appearance will be once it is dead, what temperature it died at and how long it can go with out water. We will also study it with a microscope to familiarize ourselves with its aspects while alive

16. TESTING SCHEDULE DateEvent October 10, 2010 (Sun)Structure Testing October 13, 2010 (W)Structure Testing October 17, 2010 (Sun)Testing: Payload and Function October 20, 2010 (W)Testing: Function and Cooler October 24, 2010 (Sun)Testing: Payload and Revision October 25, 2010 (M)Finish Testing

17. EXPECTED RESULTS We expect that the algae sample that is pressurized and kept relatively warm will survive the extreme conditions best. The sample that is unpressurized and exposed to the extreme cold conditions is expected to fare the worst. Algae samples that did not survive are expected to exhibit a brown color and a lack of cellular movement.

18. TEAM ORGANIZATION CHART TaskLeadSecondary Management Daniel Sayle Science Patrick ByrneDaniel Sayle Design Daniel SaylePatrick Byrne Budget Sarah KempDaniel Sayle Technical Sarah KempKatie Thompson Construction Katie ThompsonAnna McLeland Testing Katie ThompsonAnna McLeland Documentation Anna McLelandSarah Kemp

19. BIGGEST WORRIES Air Depletion Lack of gases for algae to survive off UV Radiation Avoiding the mutation and death of the algae Temperature Extreme temperatures may cause algae to die Landing Impact Limiting damage to algae samples