1. Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE) *Upstream Promoter Element

2. Transcription Initiation in Eukaryotes General Transcription Factors and the Pre-initiation complex:

3. Transcription Initiation in Eukaryotes TBP binding:

4. Transcription Initiation in Eukaryotes

6. Schematic Model Elongation of Transcription in Eukaryotes

7. Polyadenylation *CstF *CPSF *PAP *PABP RNA processing in eukaryotes Addition of poly A tail Addition of 5’cap Splicing

8. Torpedo model for transcription termination

9. Formation of the 5’ cap *RNA triphosphatase *guanylyl transferase *methyl transferase Transcript Processing

10. Functions of the Cap and Tail Protection Translatability Transport Splicing