1. DIET AND HDL COMPOSITION Michelle Averill PhD….

2. HDL Proteome and Diet Date Independent Identification of all Proteins Detectable on HDL Diet Study – 80% calories from Carbohydrate or Lipid Test Beverage – Randomized Crossover Design – Fasting, 3 hours, and 6 hours post meal – N=16

3. UTILITY OF LARGE COHORT DATA Michelle Averill PhD RD and Chris Delaney PhD

4. Cohort A cardiovascular disease cohort Michelle Averill and Chris Delaney are both MESA investigators The same coordinating center also has other cohorts that might be options – Older adults (CHS) and HIV-infected (STTR)

5. Cohort 6,814 Participants  Race/Ethnicity 38% European-American 12% Chinese-American 28% African-American 22% Hispanic-American  Female 53%  Age Exam1 45-84 years (mean 62 yrs) Exam5 53-94 years (mean 70 yrs)

6. Distribution of participants by MESA Field Centers and Ethnicity White 574 Black 503 White 524 Black 562 White 223 Black 382 Hispanic 494 White 599 Hispanic 456 White 131 Black 155 Hispanic 536 Chinese 497 White 557 Black 302 Chinese 301 Total 6,814

7. Exams, Follow Up, & Ancillary Studies

8. MESA Questionnaires by Exam Exam 1 Jul00-Aug02 (24 months) Exam 2 Sep02-Feb04 (18 Months) Exam 3 Mar04-Sep05 (18 Months) Exam 4 Sep05-May07 (21 Months) Exam 5 Apr10-Dec11 (21 Months) N=6814N=6232N=5939N=5704N=4651 Questionnaires Personal Hx XXXXX Medical Hx XXXXX Medications XXXXX Family Hx X Sleep Hx XX A 2500 Neighborhood X Psycho-Social XXXXX Occupation XXX Phys Activity XXXX Food Freq XX

9. Some other measures in MESA Subclinical atherosclerosis – CT scans of coronary arteries – IMT scans of carotid arteries – MRI scans of the heart Cardiovascular events Blood and urine biomarkers Genetics Cognitive function

10. Questions…