1. PRACTICAL PHARMACOLOGY-1 (303) Third Year FACULTY OF PHARMACY Department of Pharmacology & Toxicology CAIRO UNIVERSITY شعارالكلية منارة التعليم الصيدلي في مصر ومحيطها الإقليمي

3. Principle: Rectus abdominis is skeletal muscle : innervated by somatic motor nerve (one – neuron) as shown below: Rectus abdominis is skeletal muscle : innervated by somatic motor nerve (one – neuron) as shown below: Somatic motor nerve Nm Skeletal muscle Ach

4. Dissection: (1) Exposure of the muscle (2) Outer margin dissection (3) Inner margin dissection

5. (4) Dissected muscle (5) Tying the muscle from both ends & isolate it

6. Organ bath Air tube Suspended tissue Thread Setup:

8. Ach addition to isolated rectus abdominis  muscle contraction  pulls the lever & a line is drawn on the paper of the rotating drum. Ach addition Stop & Wash Wash 3 times till reach normal level

9. Ach Typical tracing of contractions obtained with different doses of Ach on frog rectus abdominis. 0.10.20.4 0.8

10. Dose cycle: Contact Time Washing Time Normal Time Dose Cycle Dose Cycle = Contact Time + Washing Time + Normal Time Dose cycle should be constant all over the experiment : Normal, contact & washing times should be constant

11. 0.10.20.40.8 Base line 1.6 Normal Completion of D/R: Increase Ach dose till maximum response is reached (all receptors fully occupied). Max.

12. How to measure the response of each dose: Base Line B A Response = A - B

13. Tabular & graphical presentation of data: Dose (ml) Response Peak height (mm) AB 0.1 XX 0.2 XX 0.4 XX 0.8 XX 1.6 XX A B Compare between two solutions with respect to their potencies.

15. Able to deliver the scientific & experimental concepts with: 1.Animal Saving 2.Cost Saving 3.Time Saving 4.Environmental health & safety 5.Attractive & Exciting

16. Lack of practical skills

19. 29112014

20. 29 11 2014 Type code Select Lab time

21. 29 11 2014

22. Type your name Type your ID Press Start

25. Press Add Dose

26. 25

27. Complete the D/R relationship for the given TWO Ach solutions till max. response

29. Press Select all

30. Note: If not all rows are required, select the required row by double clicking it

31. Press

33. Press printer icon to print

34. توزيع مجموعات الطلاب في امتحان نظري العملي اليومالمكانأرقام الطلابالساعة الإثنين 12/22 مـــــدرج منصور خليفة 640 - 511 3:00 – 3:10 770 – 641 3:30 – 3:40 900 - 771 4:00 – 4:10 للآخر- 901 4:30 – 4:40 الخميس 12/25 1 - 13011:00 – 11:10 131 - 26011:30 – 11:40 261 - 39012:00 – 12:10 391 - 51012:30 – 12:40 على الطلبة الباقون للإعادة حضور امتحان نظري العملي في مجموعتهم ( 901 – للآخر)