1. By: Samantha Cooney and Joshua McKain

2. Water quality differences are known to affect macro invertebrate diversity Phosphate levels are known to spike in Fernan Lake Could disequilibrium between phosphorus and nitrogen in the lake affect macro invertebrate diversity in lake outlet?

3. Initial Question Do high phosphate concentrations affect macro invertebrate biodiversity in riffles? Hypothesis If phosphate levels are high, macro invertebrate biodiversity will be low.

4. Disturb a.5 square meter area riffle with shovel Collect macro inverts with a kick net Organize inverts based on high quality, medium quality, and low quality species Collect pH, DO, water temp., air temp, and phosphorous concentration at invert collection sites Draw correlations using Anova

5. Shovel Kick net 4 sample tubes Lab quest Probes: DO, pH, temp Bug viewer Hach kit Forceps Dichotomous keys

7. Site 1 and 2 Site 4 and 5 Site 3

18. LocationAir Temp (°C)Water Temp (°C)DO (mg/L)"Phosphorus"pH 118158.9227.8 21414.29.3327.6 3208.57.937.8 41910.47.72 5209.39.507.5 AVERAGE18.211.488.671.87.68 variance6.28.6470.68021.20.017 sd2.489982.9405780.8247421.0954450.130384

19. Location High Quality Species# Species Moderate Quality Species# Species Low Quality Species# Species 1225411 2005334 3113233 4120012 5571133

20. Site 4 had low diversity and pop. density because there was no water flowing the day before we collected our samples.

21. Anova: Single Factor SUMMARY GroupsCountSumAverageVariance phospho r591.81.2 high quality species5122.47.3 ANOVA Source of VariationSSdfMSFP-valueF crit Between Groups0.91 0.21176 5 0.65763 7 5.31765 5 Within Groups3484.25 Total34.99 Analysis of the Variance to find if the means are correlated (Ho: µ1=µ2) (Ha: µ1≠µ2). f* is not in the critical region, there for we accept (Ho: µ1=µ2) and reject the null hypothesis (Ha) that the means are not related. P-value 0.657637> 0.05 therefore we accept Ho

22. Mirror Adventure Learning Place-based learning and field trips More time collecting data on inverts Lessons on dichotomous keys Blogging AL vs. DL

23. Data supported hypothesis Phosphate concentrations seem to have an affect on macro invertebrate diversity Difference of means are related Too little data may have skewed results More time in data collection More time identifying individual species Confusion with units for phosphate