1. advancing the state of the art in microscopy Imaging Protein Crystals and Managing the Associated Data

2. What Type of Microscope to Use?

3. –Sample Liquid Drop w/ Solid and/or Semi Solid

4. What Type of Microscope to Use? –Sample Liquid Drop w/ Solid and/or Semi Solid –Sample Location MicroBatch, Sitting Drop or Hanging Drop

5. Inverted Configuration Light Source Sitting Drop Or Microbatch Drop Microscope

6. Upright Configuration Microscope Hanging Drop Light Source

7. UprightInverted Sitting Drop Imaging Comparison

8. What Type of Microscope to Use? –Sample Liquid Drop w/ Solid and/or Semi Solid –Sample Location MicroBatch, Sitting Drop or Hanging Drop –Illumination Koehler

9. Properties of a Drop - Lens Effect

10. Properties of a Drop - Lens Effect - Side Wall/Refraction Ring

11. Less than 90º Contact Angle of a Drop

12. Hemispherical

13. Contact Angle of a Drop Greater than 90º

14. High Contrast = More Detail

15. Crystals at the Drop Edge - use medium contrast

16. Very dark side-wall effect - use low contrast

17. Focusing on condensation

18. Focusing on Crystals

19. Focusing on condensation Focusing on Crystals Low Contrast High Contrast

20. L.I.M.S. Laboratory Information Management System Data Management

21. Barcodes

22. Crystallization Data Experiment Details

24. Crystallization Data Experiment Details Image Management Scoring Images

29. Experiment Details Image Management Scoring Images Reviewing Results and Trends Crystallization Data

33. Experiment Details Image Management Scoring Images Reviewing Results and Trends Remote Viewing Crystallization Data

40. Experiment Details Image Management X-Ray Data

43. Experiment Details Image Management X-Ray Data Printing Results Crystallization Data