1. magnificationmagnification Copyright © 2010 Ryan P. Murphy

2. The act of expanding something in apparent size Copyright © 2010 Ryan P. Murphy

3. The act of expanding something in apparent size

4. Copyright © 2010 Ryan P. Murphy The act of expanding something in apparent size

5. Magnification: The act of expanding something in apparent size. Magnification: The act of expanding something in apparent size. – The object doesn’t change in size.

6. DE-MAGNIFICATION To make something smaller in appearance Copyright © 2010 Ryan P. Murphy

7. DE-MAGNIFICATION To make something smaller in appearance

8. Copyright © 2010 Ryan P. Murphy DE-MAGNIFICATION To make something smaller in appearance

9. How is magnification useful? Copyright © 2010 Ryan P. Murphy

10. Magnifying glass

11. Copyright © 2010 Ryan P. Murphy Eye glasses

12. To see very small things Copyright © 2010 Ryan P. Murphy

13. Surgical and medical applications Copyright © 2010 Ryan P. Murphy

14. Crime investigation – Two different bullets shot from the same gun shown below. Copyright © 2010 Ryan P. Murphy

15. Crime investigation – Two different bullets shot from the same gun shown below.

16. Which of the bills below is counterfeit? Copyright © 2010 Ryan P. Murphy

17. Which of the bills below is counterfeit?

18. Military use Copyright © 2010 Ryan P. Murphy

19. Space exploration (telescope) Copyright © 2010 Ryan P. Murphy

20. The Movies Copyright © 2010 Ryan P. Murphy

21. Cameras and recording devices. Copyright © 2010 Ryan P. Murphy

22. Magnification works because of light. Without light, you would not be able to see any image, magnified or not Copyright © 2010 Ryan P. Murphy

23. Activity! Hand Lens – Use a hand lens to practice focusing on a US $1 dollar bill. Record two pictures of neat things that you find. – Try and find the hidden owl. Copyright © 2010 Ryan P. Murphy

25. Picture of microprint on 20$ Bill. Copyright © 2010 Ryan P. Murphy

26. Convex lens: A convex lens bends the light that goes through it toward a focal point. The light spreads out again past this focal point. e e Copyright © 2010 Ryan P. Murphy

27. Convex lens: Image is reversed e e Copyright © 2010 Ryan P. Murphy

29. Although magnification is possible with only one lens, it also can be achieved by using more than one. Copyright © 2010 Ryan P. Murphy

30. Magnifying lens uses a single lens to magnify the specimen. Focusing can occur by moving the object or your eye. Copyright © 2010 Ryan P. Murphy

31. Which microscope should be used to view a bumble bee, living cell, and deep into a dead cell? Copyright © 2010 Ryan P. Murphy

32. Which device should we use to look at the specimen on the left? Copyright © 2010 Ryan P. Murphy

33. The device in the middle, called a stereoscope is used for large objects? Copyright © 2010 Ryan P. Murphy

34. Which device should we use to look at the specimen on the left? Copyright © 2010 Ryan P. Murphy

35. The microscope on the right is a compound light microscope and is used for very small specimens that light can pass through. Copyright © 2010 Ryan P. Murphy

36. It looks at things in which light cannot pass through (e.g. a bumble bee) Lets you see the image in 3D Copyright © 2010 Ryan P. Murphy

37. It lets you magnify images that light can pass through. Uses a glass slide and cover slip. Copyright © 2010 Ryan P. Murphy

39. You do not put large objects under a light microscope such a rock, pencil, finger, etc. ! Specimens need to be incredibly thin and light must pass through. Uses Slides! Copyright © 2010 Ryan P. Murphy

40. It can magnify specimens much smaller than a light, or stereoscope It doesn’t usually view live cells or specimens Copyright © 2010 Ryan P. Murphy

41. Scanning Electron Microscope Copyright © 2010 Ryan P. Murphy

42. Transmission Electron Microscope

43. Copyright © 2010 Ryan P. Murphy

70. Head of a flea Copyright © 2010 Ryan P. Murphy

72. Stapled paper

73. Copyright © 2010 Ryan P. Murphy Mascara brush

74. Copyright © 2010 Ryan P. Murphy Diatom (Protista) Shell made of glass.

75. Copyright © 2010 Ryan P. Murphy Cross section of a leaf.

76. Copyright © 2010 Ryan P. Murphy Clam Gills

77. Eye of a fruit fly.

78. Copyright © 2010 Ryan P. Murphy Variety of Pollen Grains.

79. Copyright © 2010 Ryan P. Murphy Human hair.

80. Copyright © 2010 Ryan P. Murphy Toilet Paper

81. Copyright © 2010 Ryan P. Murphy Electric Guitar String

82. Copyright © 2010 Ryan P. Murphy Avian Flu Virus

83. Copyright © 2010 Ryan P. Murphy Nylon stockings

84. Copyright © 2010 Ryan P. Murphy Microorganisms on a sheet of paper.

87. Aquatic skin parasites on fish.

88. Reptile Scales

89. Copyright © 2010 Ryan P. Murphy Microscopic Spider

91. Copyright © 2010 Ryan P. Murphy Dust mite. If you are allergic to dust, it is most likely the feces of the dust mite.

95. Porcupine quill

96. Claw of Black Widow Spider

97. Copyright © 2010 Ryan P. Murphy An atomic force microscope lets you see all the way to the atom.

98. Copyright © 2010 Ryan P. Murphy

100. When carrying a microscope, carry it by the arm, and have one hand under the base. Copyright © 2010 Ryan P. Murphy

101. Watch out for cords that hang off of the table waiting to be stepped on and pulling the microscope to the ground. Wrap the cord around arm for storage.

102. Always lower the stage after use so the gears are not strained. – Remove any slide as well. – The finely tuned gears are what make microscopes expensive. Copyright © 2010 Ryan P. Murphy

103. Remember dust cover for proper storage. Copyright © 2010 Ryan P. Murphy

104. The eyepiece lens is 10x – That means it magnifies the object ten times  Low power is usually 4x  Medium power is usually 10x  High power is usually 40x Copyright © 2010 Ryan P. Murphy

105. How many times larger is an image magnified under low power. Eye Piece x Low Power Lens = Copyright © 2010 Ryan P. Murphy

106. How many times larger is an image magnified under low power. Eye Piece x Low Power Lens = 10 x 4 = Copyright © 2010 Ryan P. Murphy

107. How many times larger is an image magnified under low power. Eye Piece x Low Power Lens = 10 x 4 = 40 magnification Copyright © 2010 Ryan P. Murphy

108. How many times larger is an image magnified under medium power and high power. Eye Piece x Medium Power Lens = 100 times magnification Copyright © 2010 Ryan P. Murphy

109. How many times larger is an image magnified under medium power and high power. Eye Piece x High Power Lens = 400 times magnification Copyright © 2010 Ryan P. Murphy

110. Create three circles using a Petri-dish.  Label the circles, low, medium and high power Copyright © 2010 Ryan P. Murphy

111. To focus the microscope…  place the slide under the stage clips and adjust the diaphragm so light passes through. o Turn revolving nose piece to the low power lens. High Power  Copyright © 2010 Ryan P. Murphy

112. To focus the microscope… image comes into focus.  With the low power lens, gently turn the coarse focus until the image comes into focus. o Once in focus, you can now use the revolving nosepiece to move the medium power lens into position.

113. Copyright © 2010 Ryan P. Murphy To focus the microscope… image comes into focus.  With the low power lens, gently turn the coarse focus until the image comes into focus. o Once in focus, you can now use the revolving nosepiece to move the medium power lens into position.

114. Copyright © 2010 Ryan P. Murphy With the medium power lens, gently turn the coarse adjustment until the image comes into focus. o Once in focus, you can now use the revolving nosepiece to move the high power lens into position.

115. Copyright © 2010 Ryan P. Murphy With the high power lens, gently turn the fine adjustment until the image comes into focus.

116. Do not use the coarse adjustment when the microscope is using the high power lens. This can break the glass slide and damage the microscope.

117. Using the microscope 1.Please place the letter e on a slide and put a cover slip on. 2.Sketch the letter e on low, medium, and high power. Copyright © 2010 Ryan P. Murphy

118. Practice your skills with prepared slides. Copyright © 2010 Ryan P. Murphy

119. Pull out a hair and put it under the microscope with a cover slip. Sketch your image. Copyright © 2010 Ryan P. Murphy

120. Activity! Using a depressed slide. – Add one drop of pond water to the depression in the slide, add a cover slip. Copyright © 2010 Ryan P. Murphy