1. Final Year Project – Seminar Presentation Azizan Mohd. Noor UniKL MICET Azizan Mohd. Noor UniKL MICET 1

2. Why do I need to do well in Presentations? Phase 1 – 35% Phase 2 – 30% Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the total marks in the assessment of the project (6 credits) comes from Presentations. Presentation is only for 30 min each For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks! WORTH YOUR WHILE TO DO WELL! Phase 1 – 35% Phase 2 – 30% Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the total marks in the assessment of the project (6 credits) comes from Presentations. Presentation is only for 30 min each For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks! WORTH YOUR WHILE TO DO WELL! 2

3. Prerequisites for a good seminar presentation Good researchGood research Good understanding of your researchGood understanding of your research Well prepared materialsWell prepared materials Good researchGood research Good understanding of your researchGood understanding of your research Well prepared materialsWell prepared materials 3

4. Preparation of seminar materials Suitable for the time allocated ie. 20 min presentation and 10 min Q&ASuitable for the time allocated ie. 20 min presentation and 10 min Q&A Suitable medium for presentation – Powerpoint being the usual choiceSuitable medium for presentation – Powerpoint being the usual choice Precise and concisePrecise and concise Suitable for the time allocated ie. 20 min presentation and 10 min Q&ASuitable for the time allocated ie. 20 min presentation and 10 min Q&A Suitable medium for presentation – Powerpoint being the usual choiceSuitable medium for presentation – Powerpoint being the usual choice Precise and concisePrecise and concise 4

5. Content of a seminar presentation IntroductionIntroduction Objective(s)Objective(s) Materials and methodsMaterials and methods Results and discussionResults and discussion ConclusionConclusion IntroductionIntroduction Objective(s)Objective(s) Materials and methodsMaterials and methods Results and discussionResults and discussion ConclusionConclusion 5

6. Introduction A brief description of the background of the researchA brief description of the background of the research Current status of knowledge in the area of researchCurrent status of knowledge in the area of research Importance of the researchImportance of the research A brief description of the background of the researchA brief description of the background of the research Current status of knowledge in the area of researchCurrent status of knowledge in the area of research Importance of the researchImportance of the research Intro1 6

7. An intracellular storage material accumulated by a wide variety accumulated by a wide variety of microorganisms of microorganisms An intracellular storage material accumulated by a wide variety accumulated by a wide variety of microorganisms of microorganisms O C (CH) n (CH 2 ) n C O 100-30 000 R Poly(3-hydroxyalkanoate) PHA Intro2 7

8. PHA can be divided into: Short-chain-length PHA (scl-PHA) eg: 3-hydroxypropionate, 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate Medium-chain-length (mcl-PHA) eg: 3-hydroxyhexanoate, 3-hydrooctanoate, 3-hydroxydecanoate Short-chain-length PHA (scl-PHA) eg: 3-hydroxypropionate, 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate Medium-chain-length (mcl-PHA) eg: 3-hydroxyhexanoate, 3-hydrooctanoate, 3-hydroxydecanoate Intro3 8

9. The type of PHAs produced depends on: Bacteria strains Carbon sources Culture conditions Bacteria strains Carbon sources Culture conditions Intro49

10. Some uses of PHAs Degradable packaging for consumer products eg. bottles, bags, films Drug-release matrix devices Starting material for the synthesis of enantiomeric pure chemicals Paint industry – as a binder Degradable packaging for consumer products eg. bottles, bags, films Drug-release matrix devices Starting material for the synthesis of enantiomeric pure chemicals Paint industry – as a binder Intro510

11. To screen for local PHA producers To optimize the culture conditions To manipulate the molecular weight of the polymer(s) To screen for local PHA producers To optimize the culture conditions To manipulate the molecular weight of the polymer(s) Objectives 11

12. Materials and methods Main methods used Concise – use diagrams or flow-charts if possible Main methods used Concise – use diagrams or flow-charts if possible Materials n methods 112

13. Procedure Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi- synthetic liquid medium with glucose as the C- source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi- synthetic liquid medium with glucose as the C- source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Materials n methods 2 13

14. Procedure Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Materials n methods 3 14

15. Screening Growth of USM4-55 in specific medium Aerobic fermentation (shake flask) Aerobic fermentation (shake flask) Cell harvesting Cell drying (freeze-drying) GCGC polymer extraction Molecular weight analysis Procedure Materials n methods 4 15

16. Results Very improtant Concise Arranged according to the objectives of the seminar Table, graph or photo? Very improtant Concise Arranged according to the objectives of the seminar Table, graph or photo? Results 1 16

17. C/N (mol/mol) Cell dry weight (g/l) Polyester content (wt%) Polyester composition 3C4 3C6 3C10 3C8 3C12 3C12:1 3C14 10 4.50 25 22 2 2 24 26 15 2 2 10 15 3.88 26 23 2 2 24 25 10 1 1 14 20 2.92 37 14 1 1 31 32 9 9 1 1 12 30 1.60 43 19 1 1 24 30 12 1 1 13 40 1.25 50 8 8 1 1 28 32 12 1 1 18 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55 Results 2 17

18. C/N (mol/mol) Cell dry weight (g/l) Polyester content (wt%) Polyester composition 3C4 3C6 3C10 3C8 3C12 3C12:1 3C14 10 4.50 25 22 2 2 24 26 15 2 2 10 15 3.88 26 23 2 2 24 25 10 1 1 14 20 2.92 37 14 1 1 31 32 9 9 1 1 12 30 1.60 43 19 1 1 24 30 12 1 1 13 40 1.25 50 8 8 1 1 28 32 12 1 1 18 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55 Results 3 18

19. Polymer molecular weight profile Results 4 19

20. Optimisation of glucoamylase production by Aspergillus niger Results 5 20

21. Microorganism Pseudomonas sp. USM4-55 was locally isolated from the soil. It was identified by using API20E. Ability to produce biopolymer detected using Nile-Blue-A staining method and gas chromatographic analysis. Microorganism Pseudomonas sp. USM4-55 was locally isolated from the soil. It was identified by using API20E. Ability to produce biopolymer detected using Nile-Blue-A staining method and gas chromatographic analysis. Results 6 21

22. mcl-P(3HA) P(3HB) Results 7 22

23. Conclusion GC is still the better method for screening of new PHA producers USM4-55 produces both scl- and mcl-PHA USM4-55 can use both glucose and oleic acid to produce PHA Polymer accumulation is highly stimulated by nitrogen and oxygen limitation Polymer molecular weight can be manipulated by adding certain chemical compounds GC is still the better method for screening of new PHA producers USM4-55 produces both scl- and mcl-PHA USM4-55 can use both glucose and oleic acid to produce PHA Polymer accumulation is highly stimulated by nitrogen and oxygen limitation Polymer molecular weight can be manipulated by adding certain chemical compounds 23

24. Effective seminar presentation Have a good understanding of the research carried out Confidence and poise Good English Address the audience Adhere to the time allocated Practise!! Have a good understanding of the research carried out Confidence and poise Good English Address the audience Adhere to the time allocated Practise!! 24