1. Lecture 35 12/2/05

2. Mobile phase Volume flow rate mL solvent/minute travle Linear flow rate cm of column length/min

3. V r = retention volume = volume of mobile phase needed to elute specific compound

6. What does a good chromatogram look like?

7. Resolution: Degree of separation between peaks

8. Band broadening caused by diffusion

9. Broadening before and after column Variance is additive

10. Column efficiency Quantitative measure of band broadening Plate height and number of plates

11. Plate Height x = distance traveled van Deemter equation

12. Open tubular columns = 0