1. BioChip Arrayer Appendix I1 - BC Array Designer NEW OPEN SAVE ARROW ASPIRATE 4 WELLS ASPIRATE MANY WELLS ZOOM ADD RACK DELETE RACK ADD LOOP PROPERTIES DELETE SPLIT DECK RULE MAPPING

2. BioChip Arrayer Using the BC Array Designer Create a new map

3. BioChip Arrayer Using the BC Array Designer Left side - represents the procedure tree Right side - represents the BCA deck

4. BioChip Arrayer Using the BC Array Designer Helpful to split the deck so that different zoom percentages can be used

5. BioChip Arrayer Using the BC Array Designer Add source rack and destination racks

6. BioChip Arrayer Using the BC Array Designer Select source rack and destination racks

7. BioChip Arrayer Using the BC Array Designer One source rack and one destination rack have been added

8. BioChip Arrayer Using the BC Array Designer Adjust zoom so that source wells and destination wells are easily seen Left mouse button magnifies Right mouse button demagnifies

9. BioChip Arrayer Using the BC Array Designer Zoom has been optimized

10. BioChip Arrayer Using the BC Array Designer Aspirate four wells from source plate and place into wells of destination rack

11. BioChip Arrayer Using the BC Array Designer Click on four wells of interest in source plate

12. BioChip Arrayer Using the BC Array Designer Drag individual source wells to well of choice in destination rack

13. BioChip Arrayer Using the BC Array Designer Aspirate many (in box) wells from source plate and place into wells of destination rack

14. BioChip Arrayer Using the BC Array Designer Choose number of replicates in the column and row direction

15. BioChip Arrayer Using the BC Array Designer Click wells in source plate in box format

16. BioChip Arrayer Using the BC Array Designer Drag box of wells from source rack and place in destination rack in desired location Distinguish replicates by color

17. BioChip Arrayer Using the BC Array Designer To use the Rule based mapping first select a box of wells

18. BioChip Arrayer Using the BC Array Designer Then click Rule based mapping icon in toolbar.

19. BioChip Arrayer Using the BC Array Designer Select destination rack Input replicates and number loops

20. BioChip Arrayer Using the BC Array Designer Fills destination wells from left to right starting with the top row

21. BioChip Arrayer Using the BC Array Designer File Test.map has been saved and now can be used in the test to make the array

22. BioChip Arrayer Using the BC Array Designer In BC Table Create new test On Rack definition tab use position map Select Test.map

23. BioChip Arrayer Using the BC Array Designer Notice the Source Rack and Destination rack are filled in Continue to create the test and run test

24. BioChip Arrayer ™ Flexibility in Array Design and Substrate Choice Cy3-labeled dUTP is dispensed onto HydroGel™ 3D substrate The array map is defined in the BC Array Designer Software