1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 6, part A Microbial Growth

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Microbial Growth Microbial growth = increase in number of cells, not cell size

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Temperature Minimum growth temperature Optimum growth temperature Maximum growth temperature The Requirements for Growth: Physical Requirements

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Temperature Figure 6.1

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Minimum, optimum, maximum Psychrophiles min 0, op 15,max 25 C Psychrotrophs min 0, op 20-30, max 40C Cause food spoilage Psychrotrophs

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Mesophiles op 25- 40C Pathogenic microorganisms Thermophiles op 50-60C Hyperthermophiles archea op 80C Thermophilus aquaticus

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Psychrotrophs Figure 6.2

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings pH Most bacteria grow between pH 6.5 and 7.5 Molds and yeasts grow between pH 5 and 6 Acidophiles grow in acidic environments Yeast and molds has lower PH about 5-6. The Requirements for Growth: Physical Requirements

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings The Requirements for Growth: Physical Requirements Osmotic Pressure Hypertonic environments, increase salt or sugar, cause plasmolysis Extreme or obligate halophiles require high osmotic pressure Facultative halophiles tolerate high osmotic pressure In Hypotonic solution water tends to inter the cell cause cell lysis

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings The Requirements for Growth: Physical Requirements Figure 6.4

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Carbon (50%) Structural organic molecules, energy source Chemoheterotrophs use organic carbon sources Chemoautotrophs, photoautotrophs use CO 2 The Requirements for Growth: Chemical Requirements

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Nitrogen (14%) In amino acids, proteins Most bacteria decompose proteins Some bacteria use NH 4 + or NO 3  A few bacteria use N 2 in nitrogen fixation Sulfur (S+P 4%) In amino acids (cysteine and methionine), vitamins( thiamine, biotin) Most bacteria decompose proteins Some bacteria use SO 4 2  or H 2 S Phosphorus In DNA, RNA, ATP, and membranes PO 4 3  is a source of phosphorus The Requirements for Growth: Chemical Requirements

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Trace Elements Inorganic elements required in small amounts Usually as enzyme cofactors Iron, cupper, zinc, The Requirements for Growth: Chemical Requirements

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Oxygen (O 2 ) The Requirements for Growth: Chemical Requirements obligate aerobes Faultative anaerobes Obligate anaerobes Aerotolerant anaerobes Microaerophiles

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Singlet oxygen: O 2 boosted to a higher-energy state Superoxide free radicals: O 2  Peroxide anion: O 2 2  Hydroxyl radical (OH  ) Toxic Forms of Oxygen

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Aerotolerant has SOD Microaerophiles Organic Growth Factors Organic compounds obtained from the environment Vitamins, amino acids, purines, pyrimidines The Requirements for Growth: Chemical Requirements

17. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Culture Medium: Nutrients prepared for microbial growth Sterile: No living microbes Inoculum: Introduction of microbes into medium Culture: Microbes growing in/on culture medium Culture Media

18. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Complex polysaccharide Used as solidifying agent for culture media in Petri plates, slants, and deeps Generally not metabolized by microbes Liquefies at 100°C Solidifies ~40°C Agar

19. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Chemically Defined Media: Exact chemical composition is known Complex Media: Extracts and digests of yeasts, meat, or plants Nutrient broth Nutrient agar Culture Media

20. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Culture Media Table 6.2 & 6.4

21. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Reducing media Contain chemicals (thioglycollate) that combine O 2 Heated to drive off O 2 Anaerobic Culture Methods

22. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Anaerobic jar Anaerobic Culture Methods Figure 6.5

23. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Anaerobic chamber Anaerobic Culture Methods Figure 6.6

24. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Sodium carbonate & sodium borohydride + water Produced Hydrogen and CO2 Palladium catalest combines oxgyen with Hydrogen produced H2O

25. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Candle jar CO 2 -packet Capnophiles require high CO 2 Figure 6.7

26. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Suppress unwanted microbes and encourage desired microbes. MacConkey Sabourauds BSA Selective Media Figure 6.9b, c

27. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Make it easy to distinguish colonies of different microbes. Blood agar Mannitol Salt Agar MacConkey Differential Media Figure 6.9a

28. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Encourages growth of desired microbe Assume a soil sample contains a few phenol- degrading bacteria and thousands of other bacteria Inoculate phenol-containing culture medium with the soil and incubate Transfer 1 ml to another flask of the phenol medium and incubate Only phenol-metabolizing bacteria will be growing Enrichment Media

29. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings A pure culture contains only one species or strain A colony is a population of cells arising from a single cell or spore or from a group of attached cells A colony is often called a colony-forming unit (CFU)

30. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Streak Plate Figure 6.10a, b