1. Hadar Less Frontiers in Metabolomics Integration of Metabolomics & Transcriptomics

2. Information Flow DNAmRNAProtein Reaction

3. Integration: The whole is larger then the sum of its parts. The combining and coordinating of separate parts or elements into a unified whole.

4. The Holy Grail Identify function of unknown gene Identify function of unknown gene Identify unknown metabolite Identify unknown metabolite Cluster genes & metabolites (pathways) Cluster genes & metabolites (pathways)

5. Biological Drawbacks Several genes encode the same enzyme Several genes encode the same enzyme One enzyme work on different substrates One enzyme work on different substrates One substrate participate in different reactions One substrate participate in different reactions

6. Technical Drawbacks Information gap between mRNA to reactions Information gap between mRNA to reactions Measuring snapshots instead of dynamics Measuring snapshots instead of dynamics Low variable to observation ratio Low variable to observation ratio

7. Current publications

8. Vision

9. Infrastructure

17. What can we do? genes compression G1 Gn E1

18. What can we do? Branch point analysis M1 M2 M5 M3 M4M6 M8M7 E12 E25 E68E67 E45 E34E36 Branch point enzymes

19. What can we do? Imitate dynamics ratio time + + + + + + + +

20. What can we do? Construct public datasets

21. Integration of Metabolomics & Transcriptomics Hadar Less Frontiers in Metabolomics