1. Polymerase Chain Reaction PCR

2. “PCR is one of those inventions like the internet, once you have used it, you cannot quite understand how people managed before it existed”

3. Nobel Prize 1993 Invented by Dr. Kary Mullis, –Research Scientist, Perkin Elmer Cetus Corporation in April 1983.

4. What is PCR? A method of “amplifying” specific DNA sequences. A test tube system for DNA replication that allows a “target” DNA sequence to be selectively amplified several million fold in just a few hours.

5. How Does PCR Work? http://www.abpischools.org.uk/resources/p oster-series/pcr/pcranim.asphttp://www.abpischools.org.uk/resources/p oster-series/pcr/pcranim.asp

6. HOW DOES PCR WORK ?

7. PCR Reaction: Template DNA Primers dNTPs Buffer Taq DNA polymerase

8. PCR Applications Endless.

9. Problems with PCR To be discussed……. Example: Bias –GC rich sequences tend to be preferentially amplified over AT rich sequences –Other sources of bias?

10. Discussion Paper #1: Saiki et al. (Mullis) Science Paper #2: Bruce et al. –Presented by Mary Beth Paper #3: Abdulmajwood et al. Paper #4: Wang and Wang