1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 5, part C Microbial Metabolism

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Chemotrophs Use energy from chemicals. Chemoheterotroph Energy is used in anabolism. Glucose Pyruvic acid NAD + NADH ETC ADP + PATP

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Chemotrophs Use energy from chemicals. Chemoautotroph, Thiobacillus ferroxidans Energy used in the Calvin-Benson cycle to fix CO 2. 2Fe 2+ 2Fe 3+ NAD + NADH ETC ADP + PATP 2 H +

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Phototrophs Use light energy. Photoautotrophs use energy in the Calvin-Benson cycle to fix CO 2. Photoheterotrophs use energy. Chlorophyll Chlorophyll oxidized ETC ADP + PATP

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Metabolic Diversity Among Organisms Nutritional typeEnergy sourceCarbon sourceExample PhotoautotrophLightCO 2 Oxygenic: Cyanobacteria plants. Anoxygenic: Green, purple bacteria. PhotoheterotrophLightOrganic compounds Green, purple nonsulfur bacteria. ChemoautotrophChemicalCOIron-oxidizing bacteria. ChemoheterotrophChemicalOrganic compounds Fermentative bacteria. Animals, protozoa, fungi, bacteria.

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Polysaccharide Biosynthesis Metabolic Pathways of Energy Use Figure 5.28

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Lipid Biosynthesis Metabolic Pathways of Energy Use Figure 5.29

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Amino Acid and Protein Biosynthesis Metabolic Pathways of Energy Use Figure 5.30a

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Amino Acid and Protein Biosynthesis Metabolic Pathways of Energy Use Figure 5.30b

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Purine and Pyrimidine Biosynthesis Metabolic Pathways of Energy Use Figure 5.31

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Are metabolic pathways that have both catabolic and anabolic functions. Amphibolic pathways Figure 5.32.1

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Amphibolic pathways Figure 5.32.2