1. Network Challenges: l Increase usage/awareness of high tech equipment l Lack of continuity, post docs, etc. - long term l Aim to create a Centre (of Excellence? or similar) l Set an ultimate (pie-in-the-sky) (bio?) goal l Led by a prominent (bio?) driver l Using fluorescence as a tool towards this goal employing:  Sensitivity (single photon/molecule) Single molecule through to bulk/real systems  Exploit the multi-dimensional nature ( ex, em, time, polarisation, …) l Bring together the strengths identified through this Network Synthesis Techniques Problems - bio, chemical, physical

2. Challenges cont…. l Synthesis (polymers & quantum dots) l Biotoxicity High  f l Low dark states (blinking)/stability - surface modification l Characterisation of probes l Multi-photon cross-sections l Fluorescence data base - qy’s, lifetimes, energy transfer parameters (Berlman)

3. Challenges cont…. l TRFIm l Complete arsenal of techniques l EWIFs  EWIF-FCS  Time-resolved EWIFS  Time-resolved EWIF anisotropy  Energy transfer  Time-resolved EWIF microscopy l NIR emission l CARS microscopy