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Polymerase Chain Reaction - PCR The photocopier of molecular biology.

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Presentation on theme: "Polymerase Chain Reaction - PCR The photocopier of molecular biology."— Presentation transcript:

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4 Polymerase Chain Reaction - PCR The photocopier of molecular biology

5 Microbes and Agriculture The Nitrogen Cycle

6 Ruminants

7 DNA Into Plant Cells

8 An example of a type of DNA fingerprinting PCR using universal eubacterial primers (8F and 1492R) “clean” community DNA vector insert Cloning reaction Transformation into competent E. coli cells Digestion with restriction enzymes

9 Lad 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 Lad

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