1. Advanced molecular genetics 224

2. Instructor e.Mail me to set an appointment Nohakhalifa@hotmail.com Office, Botany Department Room #239 in the first floor

3. Course contents - The history of DNA discovery - Introduction to classic genetics - Introduction to molecular genetics - DNA structure and function - What is the gene? - Replication, transcription and translation processes in prokaryotes and eukaryotes. - RNA and protein - Gene expression - Molecular genetic techniques -Genetic engineering and DNA cloning

4. Course requirements Weekly in class quizzes Take home assignments ( a assignemnts) Midterm exam ( for lecture and lab) Final exam ( for lecture and lab) Oral presentation Attendance

5. Lab session -Biostatistics - Data presentation in your lab report - ( making graphs and tables)

6. Lab session -DNA Isolate DNA and determine its concentration. - Analysis of DNA using gel electrophoresis. - DNA amplification using PCR techniques - DNA sequencing ( classic and modern methods) -Restriction enzymes and restriction map.

7. Lab session Protein - Isolation and analysis - Electrophoresis and concentration determination - Western technique

8. Lab session Genetic engineering - DNA cloning and plasmid design - Transformation and competent cell preparation -

9. Lab session -Oral presentations

10. Introduction of some concepts

11. Micro and macro molecules Micro-molecules: Glucose, amino acids ….etc. Macro-molecules DNA, RNA and proteins

15. Isolation of macromolecules Break the cell open Break the cell open This process is called cell lysis This process is called cell lysis Cells must be placed in a buffer solution during the lysis process. why? Cells must be placed in a buffer solution during the lysis process. why?

16. Isolation of macromolecules

17. Hierarchy & Protein Function

18. Protein Folding II

19. Regulation of Protein Function by Degradation

20. electrophoresis

21. SDS-PAGE

22. Introduction to genetics

23. What is the genetic material

24. S and R bacterial cells Streprococcus pneumonia

27. Hershy – Chase “ blender ” experiment using T2 bacteriophage

28. Bacteriophage

30. How genes are transmitted from parents to offspring?

31. Classical or mendelian genetics Gregory Mendel experiment on Garden peas 1856-1863 Gregory Mendel experiment on Garden peas 1856-1863

32. Classical genetics depends on: Phenotype: Phenotype: color, size, texture … etc. color, size, texture … etc. Genotype: Genotype: The genetic make up of the organism. The genetic make up of the organism.

33. Inheritance of seed texture Morphological phenotype W is dominant over the w

34. Inheritance of seed texture Molecular phenotype

35. Mendel first experiment Inheritance of Genetic trait(s)

36. Mendel first experiment reciprocal cross

37. Results of Mendel first experiment

39. Explanation of 3 dominant :1 recessive inheritance Monohybrid cross Monohybrid cross Independent assortment Independent assortment Segregation Segregation

40. The principal of the 3:1 inheritance Phenotype: Genotype: