1. BIOE 109
Summer 2009
Lecture 11-Part II
Speciation

2. What is speciation?

3. What is speciation?
• in Darwin’s words, speciation is the “multiplication of species”.

4. What is speciation?
• in Darwin’s words, speciation is the “multiplication of species”.
• according to the BSC, speciation occurs when populations evolve reproductive isolating mechanisms.

5. What is speciation?
• in Darwin’s words, speciation is the “multiplication of species”.
• according to the BSC, speciation occurs when populations evolve reproductive isolating mechanisms.
• the barriers may act to prevent fertilization – this is pre-zygotic isolation.

6. What is speciation?
• in Darwin’s words, speciation is the “multiplication of species”.
• according to the BSC, speciation occurs when populations evolve reproductive isolating mechanisms.
• the barriers may act to prevent fertilization – this is pre-zygotic isolation.
• may involve changes in location or timing of breeding, or courtship.

7. What is speciation?
• in Darwin’s words, speciation is the “multiplication of species”.
• according to the BSC, speciation occurs when populations evolve reproductive isolating mechanisms.
• the barriers may act to prevent fertilization – this is pre-zygotic isolation.
• may involve changes in location or timing of breeding, or courtship.
• barriers also occur if hybrids are inviable or sterile – this is post-zygotic isolation.

8. Modes of Speciation

9. Modes of Speciation
1. Allopatric speciation

10. Modes of Speciation 1. Allopatric speciation
1. Allopatric speciation
• reproductive isolation occurs in complete geographic isolation.

11. Modes of Speciation 1. Allopatric speciation
1. Allopatric speciation
• reproductive isolation occurs in complete geographic isolation (no gene flow).

12. Geographic isolation can rise from dispersal or vicariance

13. Modes of Speciation 1. Allopatric speciation
1. Allopatric speciation
• reproductive isolation occurs in complete geographic isolation (no gene flow).
Example: Hawaiian Drosophila

14. Hawaiian
Drosophila
D. suzukii
D. microthrix
D. nigribasis

15. Speciation by island-hopping

16. Modes of Speciation
2. Parapatric speciation

17. Modes of Speciation 2. Parapatric speciation
2. Parapatric speciation
• reproductive isolation occurs without complete geographic isolation (some gene flow).

18. Modes of Speciation 2. Parapatric speciation
2. Parapatric speciation
• reproductive isolation occurs without complete geographic isolation (some gene flow).
Example: ring species of salamanders (Ensatina) in CA

19. Ensatina salamanders

20. Ring species – evidence for parapatric speciation

21. Ring species – evidence for parapatric speciation

22. Modes of Speciation
3. Sympatric speciation

23. Modes of Speciation 3. Sympatric speciation
3. Sympatric speciation
• reproductive isolation evolves with complete geographic overlap.

24. Modes of Speciation 3. Sympatric speciation
3. Sympatric speciation
• reproductive isolation evolves with complete geographic overlap.
Example: the apple maggot fly, Rhagoletis pomonella?

25. Apple maggot fly Hawthorn fly
Speciation due to host specialization in this case

26. Evolution of reproductive
Modes of speciation: summary
Allopatric
peripatric
parapatric
sympatric
Original population
Initial step of speciation
Barrier
formation
New
niche
New
niche
Genetic
polymorphism
Evolution of reproductive
isolation
In isolation
In isolation
In adjacent
niche
Within the
population

27. What evolutionary processes are involved in speciation?

28. What evolutionary processes are involved in speciation?
1. Natural selection

29. What evolutionary processes are involved in speciation?
1. Natural selection
• driven by different abiotic conditions (e.g., temperature, altitude) and biotic conditions (e.g., competitors, parasites).

30. What evolutionary processes are involved in speciation?
1. Natural selection
• driven by different abiotic conditions (e.g., temperature, altitude) and biotic conditions (e.g., competitors, parasites).
2. Sexual selection

31. What evolutionary processes are involved in speciation?
1. Natural selection
• driven by different abiotic conditions (e.g., temperature, altitude) and biotic conditions (e.g., competitors, parasites).
2. Sexual selection
• both female choice and male-male competition can promote rapid divergence (e.g., Hawaiian Drosophila).

32. What evolutionary processes are involved in speciation?
1. Natural selection
• driven by different abiotic conditions (e.g., temperature, altitude) and biotic conditions (e.g., competitors, parasites).
2. Sexual selection
• both female choice and male-male competition can promote rapid divergence (e.g., Hawaiian Drosophila).
• sexual antagonistic selection too!

33. Male-male competition in Hawaiian Drosophila
Establish territory
On a lek by head
butting
Fight over display
Territory by
grappling

34. What evolutionary processes are involved in speciation?
3. Random genetic drift

35. What evolutionary processes are involved in speciation?
3. Random genetic drift
• may involve founder effects and genetic bottlenecks.

36. What evolutionary processes are involved in speciation?
3. Random genetic drift
• may involve founder effects and genetic bottlenecks.
• alleles that are neutral in one environment may not be neutral in another!

37. Some generalities
1. The magnitude of pre-zygotic and post-zygotic isolation both increase with the time.

38. Some generalities
1. The magnitude of prezygotic and postzygotic isolation both increase with the time.
• in Drosophila, it takes about 1.5 to 3 million years for complete isolation to evolve.

39. Some generalities
1. The magnitude of prezygotic and postzygotic isolation both increase with the time.
• in Drosophila, it takes about 1.5 to 3 million years for complete isolation to evolve.
• in marine bivalves, it may take 4 to 6 million years!

40. Some generalities
1. The magnitude of prezygotic and postzygotic isolation both increase with the time.
• in Drosophila, it takes about 1.5 to 3 million years for complete isolation to evolve.
• in marine bivalves, it may take 4 to 6 million years!
2. Among recently separated groups, pre-zygotic isolation is generally stronger than post-zygotic isolation.

41. Some generalities
 3. In the early stages of speciation, hybrid sterility or inviability is almost always seen in the heterogametic sex.

42. Some generalities
 3. In the early stages of speciation, hybrid sterility or inviability is almost always seen in the heterogametic sex.
• for example, D. simulans and D. mauritiana female hybrids are completely viable yet male hybrids are completely sterile!

43. Some generalities
 3. In the early stages of speciation, hybrid sterility or inviability is almost always seen in the heterogametic sex.
• for example, D. simulans and D. mauritiana female hybrids are completely viable yet male hybrids are completely sterile!
• this is called Haldane’s rule.
J.B.S. Haldane ( )

44. What causes post-zygotic isolation?

45. What causes postzygotic isolation?
• the underlying mechanism is called Dobzhansky-Muller incompatibility:  

46. Dobzhansky and Muller were incompatible!
“Balanced”
school
“Classical”
school

47. What causes postzygotic isolation?
• the underlying mechanism is called Dobzhansky-Muller incompatibility:  
Ancestral Pop: A1A1B1B1

48. What causes postzygotic isolation?
• the underlying mechanism is called Dobzhansky-Muller incompatibility:  
Ancestral Pop: A1A1B1B1
 
Derived Pops: A2A2B1B1 A1A1B2B2

49. What causes postzygotic isolation?
• the underlying mechanism is called Dobzhansky-Muller incompatibility:  
Ancestral Pop: A1A1B1B1
 
Derived Pops: A2A2B1B1 A1A1B2B2
 
Hybrids: A1A2B1B  fitness

50. Differences between plant and animal speciation

51. Differences between plant and animal speciation
• in plants, polyploidization is a major mode of speciation.

52. Differences between plant and animal speciation
• in plants, polyploidization is a major mode of speciation.
• polyploidization refers to the retention of extra sets of chromosomes (i.e., tetraploids, octoploids, etc.)

53. Differences between plant and animal speciation
• in plants, polyploidization is a major mode of speciation.
• polyploidization refers to the retention of extra sets of chromosomes (i.e., tetraploids, octoploids, etc.)
• there are two types of polyploids: autopolyploids and allopolyploids.

54. Differences between plant and animal speciation
• autopolyploids add chromosomal sets from the same species:

55. Differences between plant and animal speciation
• autopolyploids add chromosomal sets from the same species:
Species 1 x Species 1  Species 2
(2N = 4) (2N = 4) (4N = 8)

56. Differences between plant and animal speciation
• autopolyploids add chromosomal sets from the same species:
Species 1 x Species 1  Species 2
(2N = 4) (2N = 4) (4N = 8)
• allopolyploids combine chromosomal sets from different species:

57. Differences between plant and animal speciation
• autopolyploids add chromosomal sets from the same species:
Species 1 x Species 1  Species 2
(2N = 4) (2N = 4) (4N = 8)
• allopolyploids combine chromosomal sets from different species:
Species 1 x Species 2  Species 3
(2N = 4) (2N = 6) (2N = 10)

58. Secondary contact and reinforcement

59. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.

60. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.
Three outcomes are possible:

61. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.
Three outcomes are possible:
1. No interbreeding occurs

62. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.
Three outcomes are possible:
1. No interbreeding occurs
• isolating mechanisms in place – speciation completed.

63. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.
Three outcomes are possible:
1. No interbreeding occurs
• isolating mechanisms in place – speciation completed.
2. Introgression

64. Secondary contact and reinforcement
• secondary contact occurs when two formerly allopatric populations meet.
Three outcomes are possible:
1. No interbreeding occurs
• isolating mechanisms in place – speciation completed.
2. Introgression
• no isolating mechanisms in place – populations merge completely.

65. Secondary contact and reinforcement
3. Partial interbreeding occurs

66. Secondary contact and reinforcement
3. Partial interbreeding occurs
• some isolating mechanisms in place – a hybrid zone forms (but hybrids are less fit).

67. Secondary contact and reinforcement
3. Partial interbreeding occurs
• some isolating mechanisms in place – a hybrid zone forms (but hybrids are less fit).
• reinforcement should occur to “complete” the process by the evolution of additional pre-zygotic barriers.

68. Evidence for reinforcement in Drosophila

69. Evidence for reinforcement in Drosophila
• Coyne & Orr (1997) compared sister species of Drosophila that were either allopatric or sympatric.

70. Evidence for reinforcement in Drosophila
• Coyne & Orr (1997) compared sister species of Drosophila that were either allopatric or sympatric.
For each species pair they estimated:

71. Evidence for reinforcement in Drosophila
• Coyne & Orr (1997) compared sister species of Drosophila that were either allopatric or sympatric.
For each species pair they estimated:
1. The degree of pre-mating isolation from mate choice experiments.

72. Evidence for reinforcement in Drosophila
• Coyne & Orr (1997) compared sister species of Drosophila that were either allopatric or sympatric.
For each species pair they estimated:
1. The degree of premating isolation from mate choice experiments.
2. The degree of genetic divergence using allozymes.

73. Evidence for reinforcement in Drosophila

74. Ecological speciation in sticklebacks

75. Ecological speciation in sticklebacks

76. Ecological speciation in sticklebacks

77. Ecological speciation in sticklebacks

78. Ecological speciation in sticklebacks
1. Colonization by marine
stickleback ~10,000 years ago

79. Ecological speciation in sticklebacks
1. Colonization by marine
stickleback ~10,000 years ago
2. Adaptation to freshwater
environment

80. Ecological speciation in sticklebacks
1. Colonization by marine
stickleback ~10,000 years ago
2. Adaptation to freshwater
environment
3. Secondary invasion by marine
stickleback

81. Ecological speciation in sticklebacks
3. Secondary invasion by marine
stickleback

82. Ecological speciation in sticklebacks
3. Secondary invasion by marine
stickleback
4. Evolution of limnetic and
benthic sticklebacks

83. Evidence for secondary invasion hypothesis

84. Evidence for secondary invasion hypothesis
1. Only low elevation lakes possess limnetic and benthic
species pairs.

85. Evidence for secondary invasion hypothesis
1. Only low elevation lakes possess limnetic and benthic
species pairs.
2. Cores from lakes with limnetic and benthic species pairs show evidence of salt water influx (e.g, clams, etc.).

86. Evidence for secondary invasion hypothesis
1. Only low elevation lakes possess limnetic and benthic
species pairs.
2. Cores from lakes with limnetic and benthic species pairs show evidence of salt water influx (e.g, clams etc.).
3. Higher elevation lakes have neither limnetic and benthic species pairs nor evidence of salt water influx.

87. What types of genes are involved in speciation?
 Example: desat-2 in D. melanogaster

88. What types of genes are involved in speciation?
 Example: desat-2 in D. melanogaster
• D. melanogaster has radiated out of Africa with humans and lives all over the world (in our garbage cans).

89. What types of genes are involved in speciation?
 Example: desat-2 in D. melanogaster
• D. melanogaster has radiated out of Africa with humans and lives all over the world (in our garbage cans).
• female flies from Africa (A) possess a different cuticular hydrocarbon than cosmopolitan females (C).

90. What types of genes are involved in speciation?
 Example: desat-2 in D. melanogaster
• D. melanogaster has radiated out of Africa with humans and lives all over the world (in our garbage cans).
• female flies from Africa (A) possess a different cuticular hydrocarbon than cosmopolitan females (C).
• difference due to a different position of a single double bond.

91. 2. desat-2 in D. melanogaster
• the desat-2 mutation also affects mate choice.

92. 2. desat-2 in D. melanogaster
• the desat-2 mutation also affects mate choice.
• when A females are placed with A and C males, they only mate with the former.

93. 2. desat-2 in D. melanogaster
• the desat-2 mutation also affects mate choice.
• when A females are placed with A and C males, they only mate with the former.
• this modified hydrocarbon affects female smell – in effect they wear a different “perfume”.

94. 2. desat-2 in D. melanogaster
• the desat-2 mutation also affects mate choice.
• when A females are placed with A and C males, they only mate with the former.
• this modified hydrocarbon affects female smell – in effect they wear a different “perfume”.
• the A females are not courted very intensely by C males.