1. Electron Transport Chain (ETC) and Oxidative Phosphorylation (Ox Phos)

2. ETC and Ox Phos Electron Transport Chain (ETC) AKA respiratory chain importance: allows conversion of reducing equivalents to ATP location: inner mitochondrial membrane

3. Detailed Mitochondrial Structure

4. “Sites” are Used to Describe the ETC Molecules not completely known Sites I-IV Overview of the four

5. Analogy of waterline with valves at various spots Many Well-known Poisons are Poisons of the ETC

6. Site I in detail NADH FMN Fe-S CoQ 10 Know net reaction and name of “enzyme”

7. Sites II-IV Know net reactions and names of “enzymes”

8. Electron Flow per se Does not Cause ATP Synthesis

9. Q Cycle A proton gradient is developed

10. Mitchell’s Chemiosmotic Theory Proton gradient tightly coupled to ATP synthase

11. Fo- oligomycin-sensitive integral membrane portion F1- hexamer of  - and ß-subunits  -subunit estimated to rotate at 7,000 rpm ATP Synthase is an Oligomeric Rotor

12. Oligomycin others exist Inhibitors of ATP Synthase

13. 2,4-DNP others exist one way of experimentally determining importance of proton gradient Uncouplers of ATP Synthesis

14. Moles ATP Atoms oxygen Theoretically 3 (NAD) or 2 (FAD) Experimentally 2.8 is a ballpark real number P:O Ratio

15. Availability of ADP (usually) Regulation of ATP Synthesis