1. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Chapter 21 The Genetic Basis of Development 張學偉 生物醫學暨環境生物學系 助理教授 http://genomed.dlearn.kmu.edu.tw

2. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Overview: From Single Cell to Multicellular Organism Use mutations to deduce developmental pathways Figure 21.1 Extra small eye on its antenna

3. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 如何藉由某種生物來了解 ”development”

4. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Model organism The organism chosen for understand broad biological principles is called a model organism. Figure 21.2 DROSOPHILA MELANOGASTER (FRUIT FLY) CAENORHABDITIS ELEGANS (NEMATODE) 0.25 mm MUS MUSCULUS (MOUSE) DANIO RERIO (ZEBRAFISH) ARABIDOPSIS THAMANA (COMMON WALL CRESS) No human, why?

5. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The fruit fly Drosophila melanogaster was first chosen as a model organism The fruit fly is small and easily grown in the laboratory. – It has a generation time of only two weeks and produces many offspring. – Embryos develop outside the mother’s body. – Sequencing of Drosophila genome was completed in 2000.

6. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The nematode Caenorhabditis elegans normally lives in the soil but is easily grown in petri dishes. millimeter long, transparent body with only a few cell types and grows from zygote to mature adult in only three and a half days. genome sequenced. Because individuals are hermaphrodites, it is easy to detect recessive mutations. Self-fertilization of heterozygotes will produce some homozygous recessive offspring with mutant phenotypes. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings

7. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings

8. The mouse Mus musculus has a long history as a mammalian model of development. – Genome and genes roughly the same as human. – Researchers are adepts at manipulating mouse genes to make transgenic mice and mice in which particular genes are “knocked out” by mutation. – Generation time of 9 weeks, but embryo development inside mother is disadvantages for studies. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings

9. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A second vertebrate model, the zebrafish Danio rerio, has some unique advantages. – These small fish (2 - 4 cm long) are easy to breed in the laboratory in large numbers. – Transparent embryos develop outside the mother’s body. – Although generation time is two to four months, the early stages of development proceed quickly. By 24 hours after fertilization, most tissues and early versions of the organs have formed. After two days, the fish hatches out of the egg case.

10. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings For studying the molecular genetics of plant development, researchers are focusing on a small weed Arabidopsis thaliana (a member of the mustard family). – One plant can grow and produce thousands of progeny after eight to ten weeks. – A hermaphrodite, each flower makes ova and sperm. – Easy for gene manipulation research (genetic transformation) – Its relatively small genome, completely sequenced.

11. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Feedback problems What organism can be the model organism? What is the common criteria for these model organism? What is the advantages of using @ for model organism for genetic studies?

12. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 21.1: Embryonic development involves cell division, cell differentiation, and morphogenesis In the embryonic development of most organisms – A single-celled zygote gives rise to cells of many different types, each with a different structure and corresponding function

13. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The transformation from a zygote into an organism has three interrelated processes: cell division, cell differentiation, and morphogenesis Figure 21.3a, b (a) Fertilized eggs of a frog (b) Tadpole hatching from egg

14. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Through a succession of mitotic cell divisions – The zygote gives rise to a large number of cells In cell differentiation – Cells become specialized in structure and function Morphogenesis (creation of form) encompasses the processes – give shape to the organism and its various parts Morphogenesis = morphology + genesis

15. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Embryo development in plant and animal

16. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Fig. 21.4 Cell division Morphogenesis Observable cell differentiation

17. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The overall schemes of morphogenesis in animals and plants are very different. In animals, movements of cells and tissues transform the embryo. In plants, morphogenesis and growth in overall size are not limited to embryonic and juvenile periods.

18. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Apical meristems, perpetually embryonic regions in the tips of shoots and roots, are responsible for the plant’s continual growth and formation of new organs, such as leaves and roots. In animals, ongoing development in adults is restricted to the differentiation of cells, such as blood cells, that must be continually replenished.

19. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Why different cell types are found in organisms?

20. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 21.2: Different cell types result from differential gene expression in cells with the same DNA Evidence for Genomic Equivalence Nearly all the cells of an organism have genomic equivalence. that is, they have the same genes, regardless of their state of differentiation.

21. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The de-differentiated potential for plant and animal.  Totipotent vs multipotent

22. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Totipotency in Plants One experimental approach for testing genomic equivalence – Is to see whether a differentiated cell can generate a whole organism

23. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings EXPERIMENT Transverse section of carrot root 2-mg fragments Fragments cul- tured in nutrient medium; stir- ring causes single cells to shear off into liquid. Single cells free in suspension begin to divide. Embryonic plant develops from a cultured single cell. Plantlet is cul- tured on agar medium. Later it is planted in soil. RESULTS A single somatic (non- reproductive) carrot cell developed into a mature carrot plant. The new plant was a genetic duplicate (clone) of the parent plant. Adult plant CONCLUSION Figure 21.5 Can a differentiated plant cell develop into a whole plant? At least some differentiated (somatic) cells in plants are toipotent, able to reverse their differentiation and then give rise to all the cell types in a mature plant.

24. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A totipotent cell – Is one capable of generating a complete new organism Cloning – Is using one or more somatic cells from a multicellular organism to make another genetically identical individual

25. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Nuclear Transplantation in Animals In nuclear transplantation – The nucleus of an unfertilized egg cell or zygote is replaced with the nucleus of a differentiated cell

26. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Experiments with frog embryos  transplanted nucleus support normal development of egg EXPERIMENT Researchers enucleated frog egg cells by exposing them to ultraviolet light, which destroyed the nucleus. Nuclei from cells of embryos up to the tadpole stage were transplanted into the enucleated egg cells. Frog embryo Frog egg cell Frog tadpole Less differentiated cell Donor nucleus transplanted Enucleated egg cell Fully differentiated (intestinal) cell Donor nucleus transplanted Most develop into tadpoles <2% develop into tadpoles Figure 21.6

27. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings RESULTS Most of the recipient eggs developed into tadpoles when the transplanted nuclei came from cells of an early embryo, which are relatively undifferentiated cells. But with nuclei from the fully differentiated intestinal cells of a tadpole, fewer than 2% of the eggs developed into normal tadpoles, and most of the embryos died at a much earlier developmental stage. CONCLUSION The nucleus from a differentiated frog cell can direct development of a tadpole. However, its ability to do so decreases as the donor cell becomes more differentiated, presumably because of changes in the nucleus. With age increase

28. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Clone & Cloning

29. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Reproductive Cloning of Mammals In 1997, Scottish researchers – Cloned a lamb from an adult sheep by nuclear transplantation

30. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Nucleus removed Mammary cell donor Egg cell donor Egg cell from ovary Cultured mammary cells are semistarved, arresting the cell cycle and causing dedifferentiation Nucleus from mammary cell Grown in culture Early embryo Implanted in uterus of a third sheep Surrogate mother Embryonic development Lamb (“Dolly”) genetically identical to mammary cell donor 4 5 6 1 2 3 Cells fused APPLICATION This method is used to produce cloned animals whose nuclear genes are identical to the donor animal supplying the nucleus. TECHNIQUE Shown here is the procedure used to produce Dolly, the first reported case of a mammal cloned using the nucleus of a differentiated cell. RESULTS The cloned animal is identical in appearance and genetic makeup to the donor animal supplying the nucleus, but differs from the egg cell donor and surrogate mother. Nucleus removed Figure 21.7

31. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings “Copy Cat” – Was the first cat ever cloned Figure 21.8 Cute ! vs Kill ?

32. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Problems Associated with Animal Cloning In most nuclear transplantation studies performed thus far – Only a small percentage of cloned embryos develop normally to birth

33. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Stem cells ( 可以跟植物 Totipotent 相比較的細胞 )

34. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 30 MARCH 2001 VOL 291 SCIENCE Page. 2552

35. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings SCIENCE VOL 293 6 JULY 2001 page.95

36. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Stem Cells of Animals A stem cell – Is a relatively unspecialized cell – Can reproduce itself indefinitely (renewal) – Can differentiate into specialized cells of one or more types, given appropriate conditions

37. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 21.9 Early human embryo at blastocyst stage (mammalian equiva- lent of blastula) From bone marrow in this example Totipotent cells Pluripotent cells Cultured stem cells Different culture conditions Different types of Differentiated cells Liver cells Nerve cells Blood cells Embryonic Stem cells can be isolated  From early embryos at the blastocyst stage Embryonic stem cellsAdult stem cells Adult stem cells  Are said to be pluripotent, able to give rise to multiple but not all cell types

38. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Transcriptional Regulation of Gene Expression During Development Cell determination – Precedes differentiation and involves the expression of genes for tissue-specific proteins

39. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings DNA OFF Master control gene myoDOther muscle-specific genes Nucleus Embryonic precursor cell Figure 21.10 Determination and differentiation of muscle cells (layer 1)

40. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings DNA OFF mRNA MyoD protein (transcription factor) Master control gene myoD Determination. Signals from other cells lead to activation of a master regulatory gene called myoD, and the cell makes MyoD protein, a transcription factor. The cell, now called a myoblast, is irreversibly committed to becoming a skeletal muscle cell. 1 Other muscle-specific genes Nucleus Myoblast (determined) Embryonic precursor cell Figure 21.10 Determination and differentiation of muscle cells (layer 2)

41. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings DNA OFF mRNA Another transcription factor MyoD Muscle cell (fully differentiated) MyoD protein (transcription factor) Myoblast (determined) Embryonic precursor cell Myosin, other muscle proteins, and cell-cycle blocking proteins Other muscle- specific genes Master control gene myoD Nucleus Determination. Signals from other cells lead to activation of a master regulatory gene called myoD, and the cell makes MyoD protein, a transcription factor. The cell, now called a myoblast, is irreversibly committed to becoming a skeletal muscle cell. 1 Differentiation. MyoD protein stimulates the myoD gene further, and activates genes encoding other muscle-specific transcription factors, which in turn activate genes for muscle proteins. MyoD also turns on genes that block the cell cycle, thus stopping cell division. The nondividing myoblasts fuse to become mature multinucleate muscle cells, also called muscle fibers. 2 Figure 21.10 Determination and differentiation of muscle cells (layer 3)

42. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Cytoplasmic Determinants and Cell-Cell Signals in Cell Differentiation Figure 21.11a Cytoplasmic determinants in the egg. The unfertilized egg cell has molecules in its cytoplasm, encoded by the mother’s genes, that influence development. Many of these cytoplasmic determinants, like the two shown here, are unevenly distributed in the egg. After fertilization and mitotic division, the cell nuclei of the embryo are exposed to different sets of cytoplasmic determinants and, as a result, express different genes. (a) Sperm Molecules of another cyto- plasmic deter- minant Unfertilized egg cell Molecules of a a cytoplasmic determinant Fertilization Zygote (fertilized egg) Mitotic cell division Two-celled embryo Nucleus Sperm Maternal effect genes Fertilization zygote effect genes Animation:21fly

43. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In the process called induction  Signal molecules from embryonic cells cause transcriptional changes in nearby target cells Figure 21.11b Induction by nearby cells. The cells at the bottom of the early embryo depicted here are releasing chemicals that signal nearby cells to change their gene expression. (b) Signal transduction pathway Signal receptor Signal molecule (inducer) Early embryo (32 cells) NUCLEUS Animation:21signal

44. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 21.3: Pattern formation in animals and plants results from similar genetic and cellular mechanisms Pattern formation – Is the development of a spatial organization of tissues and organs – Occurs continually in plants – Is mostly limited to embryos and juveniles in animals Slide p.17

45. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Positional information – Consists of molecular cues that control pattern formation – Tells a cell its location relative to the body’s axes and to other cells

46. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Drosophila Development: A Cascade of Gene Activations Pattern formation- extensively studied in Drosophila melanogaster The Life Cycle of Drosophila The fruit fly Drosophila melanogaster was first chosen as a model organism The fruit fly is small and easily grown in the laboratory. – It has a generation time of only two weeks and produces many offspring. – Embryos develop outside the mother’s body.

47. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Key developmental events in life cycle of Drosophila Figure 21.12 Follicle cell Nucleus Egg cell Fertilization Nurse cell Egg cell developing within ovarian follicle Laying of egg Egg shell Nucleus Fertilized egg Embryo Multinucleate single cell Early blastoderm Plasma membrane formation Late blastoderm Cells of embryo Yolk Segmented embryo Body segments 0.1 mm Hatching Larval stages (3) Pupa Metamorphosis Head Thorax Abdomen 0.5 mm Adult fly Dorsal Anterior Posterior Ventral BODY AXES 2 1 3 4 5 6 7 positional information for number of correctly oriented segments.  finally each segment’s characteristic structures. positional information for the two developmental axes before fertilization. After fertilization

48. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Genetic Analysis of Early Development: Scientific Inquiry Axis Establishment Maternal effect genes – Encode for cytoplasmic determinants that initially establish the axes of the body of Drosophila

49. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Maternal effect genes Flies with the bicoid mutation – Do not develop a body axis correctly Head Wild-type larva Tail Mutant larva (bicoid) Drosophila larvae with wild-type and bicoid mutant phenotypes. A mutation in the mother’s bicoid gene leads to tail structures at both ends (bottom larva). The numbers refer to the thoracic and abdominal segments that are present. (a) T1 T2 T3 A1 A2 A3 A4 A5 A6 A7 A8 A7A6A7 A8 Tail Figure 21.14a

50. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Maternal effect gene (b) Gradients of bicoid mRNA and Bicoid protein in normal egg and early embryo. Translation of bicoid mRNA Fertilization Nurse cells Egg cell bicoid mRNA Developing egg cell Bicoid mRNA in mature unfertilized egg 100 µm Bicoid protein in early embryo Anterior end 1 2 3 Figure 21.14b Animation: 21ceembryo

51. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Segmentation Pattern Segmentation genes – Produce proteins that direct formation of segments after the embryo’s major body axes are formed

52. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Sequential activation of three sets of segmentation genes 1.Gap genes 2.Pair-rule genes 3.Segment polarity genes

53. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Gap genes map out the basic subdivisions along the anterior-posterior axis. – Mutations cause “gaps” in segmentation.

54. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Pair-rule genes define the modular pattern in terms of pairs of segments. Mutations result in embryos with half the normal segment number.

55. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Segment polarity genes set the anterior- posterior axis of each segment. Mutations produce embryos with the normal segment number, but with part of each segment replaced by a mirror-image repetition of some other part.

56. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Identity of Body Parts The anatomical identity of Drosophila segments is set by master regulatory genes called homeotic genes Figure 21.13 Eye Antenna Leg Wild type Mutant Mutations to homeotic genes  Structures characteristic of a particular part of the animal arise in the wrong place.

57. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A summary of gene activity during Drosophila development Hierarchy of Gene Activity in Early Drosophila Development Maternal effect genes (egg-polarity genes) Gap genes Pair-rule genes Segment polarity genes Homeotic genes of the embryo Other genes of the embryo Segmentation genes of the embryo

58. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings C. elegans: The Role of Cell Signaling The complete cell lineage  Of each cell in the nematode roundworm C. elegans is knownC. elegans Figure 21.15 Zygote Nervous system, outer skin, musculature Musculature, gonads Outer skin, nervous system Germ line (future gametes) Musculature First cell division Time after fertilization (hours) 0 10 Hatching Intestine Eggs Vulva ANTERIOR POSTERIOR 1.2 mm

59. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Induction As early as the four-cell stage in C. elegans  Cell signaling helps direct daughter cells down the appropriate pathways, a process called induction Figure 21.16a 4 Anterior EMBRYO Posterior Receptor Signal protein Signal Anterior daughter cell of 3 Posterior daughter cell of 3 Will go on to form muscle and gonads Will go on to form adult intestine 1 2 4 3 3 Induction of the intestinal precursor cell at the four-cell stage. (a)

60. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Induction is also critical later in nematode development  As the embryo passes through three larval stages prior to becoming an adult Figure 21.16b Epidermis GonadAnchor cell Signal protein Vulval precursor cells Inner vulva Outer vulva Epidermis ADULT Induction of vulval cell types during larval development. (b) Larva

61. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Programmed Cell Death (Apoptosis) 生時燦如夏花 死時美若秋葉 ---- 泰格爾

62. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In apoptosis – Cell signaling is involved in programmed cell death 2 µm Figure 21.17. Apoptosis of human white blood cells. normal

63. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ced-9  master regulator of apoptosis Ced-3  chief caspase, the main proteases of apoptosis Ced-9 protein (active) inhibits Ced-4 activity Mitochondrion Death signal receptor Ced-4Ced-3 Inactive proteins (a) No death signal Ced-9 (inactive) Cell forms blebs Death signal Active Ced-4 Active Ced-3 Activation cascade Other proteases Nucleases (b) Death signal Figure 21.18a, b Apoptosis is regulated not at the level of transcription or translation, but through changes in the activity of proteins that are continually present in the cell. outer mitochondrial membrane

64. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In vertebrates – Apoptosis is essential for normal morphogenesis of hands and feet in humans and paws in other animals Figure 21.19 Interdigital tissue 1 mm

65. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Plant Development: Cell Signaling and Transcriptional Regulation Thanks to DNA technology and clues from animal research – Plant research is now progressing rapidly

66. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Mechanisms of Plant Development In general, cell lineage – Is much less important for pattern formation in plants than in animals The embryonic development of most plants – Occurs inside the seed

67. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Pattern Formation in Flowers Floral meristems – Contain three cell types (L1-3) that affect flower development Figure 21.20 Carpel Petal Stamen Sepal Anatomy of a flower Floral meristem Tomato flower Cell layers L1 L2 L3 flower with four types of organs

68. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Tomato plants with a mutant allele – Have been studied in order to understand the genetic mechanisms behind flower development Researchers grafted stems from mutant plants onto wild-type plants. They then planted the shoots that emerged near the graft site, many of which were chimeras. Sepal Petal Carpel Stamen Wild-type normal Fasciated (ff) extra organs Tomato plants with the fasciated (ff) mutation develop extra floral organs. EXPERIMENT Figure 21.21 Graft Chimeras For each chimera, researchers recorded the flower phenotype: wild-type or fasciated. Analysis using other genetic markers identified the parental source for each of the three cell layers of the floral meristem (L1–L3) in the chimeras.

69. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings RESULTS CONCLUSION The flowers of the chimeric plants had the fasciated phenotype only when the L3 layer came from the fasciated parent. Cells in the L3 layer induce the L1 and L2 layers to form flowers with a particular number of organs. Wild-type Fasciated (ff Floral meristem L1 L2 L3 Key Wild-type parent Plant Flower Phenotype Floral Meristem Chimera 1 Chimera 2 Chimera 3 Wild-type Fasciated Wild-type ) Fasciated (ff) parent The number of organs per flower depends on genes of the L3 layer  organ identity genes 參考

70. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Organ identity genes – Determine the type of structure that will grow from a meristem – Are analogous to homeotic genes in animals Figure 21.22 Wild typeMutant

71. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 21.4: Comparative studies help explain how the evolution of development leads to morphological diversity Biologists in the field of evolutionary developmental biology, or “evo-devo,” as it is often called – Compare developmental processes of different multicellular organisms

72. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings All homeotic genes of Drosophila include a 180-nucleotide sequence called the homeobox, which specifies a 60-amino-acid homeodomain, part of a transcription factor. Homeobox-containing genes often called Hox genes, especially in mammals conserved in animals for hundreds of millions of years. Related sequences are present in yeast, prokaryotes, human …etc. Widespread Conservation of Developmental Genes Among Animals

73. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings An identical or very similar nucleotide sequence in the homeotic genes of both vertebrates and invertebrates Figure 21.23 Adult fruit fly Fruit fly embryo (10 hours) Fly chromosome Mouse chromosomes Mouse embryo (12 days) Adult mouse

74. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Not all, homeobox-containing genes are homeotic genes that are associated with development.  some don’t directly control the identity of body parts. For example, in Drosophila, homeoboxes are present not only in the homeotic genes but also in the egg-polarity gene bicoid, in several segmentation genes, and in the master regulatory gene for eye development.

75. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Effect of differences in Hox gene expression In some cases, small changes in regulatory sequences of particular genes can lead to major changes in body form. Thorax Genital segments Abdomen ThoraxAbdomen Figure 21.24 crustaceans insects

76. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings In other cases – Genes with conserved sequences play different roles in the development of different species In plants – Homeobox-containing genes do not function in pattern formation as they do in animals

77. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Comparison of Animal and Plant Development In both plants and animals – Development relies on a cascade of transcriptional regulators turning genes on or off in a finely tuned series But the genes that direct analogous developmental processes – Differ considerably in sequence in plants and animals, as a result of their remote ancestry