1. 03 May 2004Coventry DoughertyBiol 466H Presentation Apoptosis: Measuring the Seasonal Cycle Of Ovarian Growth & Regression in the American Crow (Corvus brachyrhynchos Coventry Dougherty Apoptosis: Measuring the Seasonal Cycle Of Ovarian Growth & Regression in the American Crow (Corvus brachyrhynchos ) Coventry Dougherty

2. 03 May 2004Coventry DoughertyBiol 466H Presentation West Nile Virus  First Reported Case westnilemaps.usgs.gov/ usa_avian.html  Transmission www.oar.noaa.gov/.../archive/spot_westnile.html

3. 03 May 2004Coventry DoughertyBiol 466H Presentation American Crow Diets  Omnivorous  Seeds and fruits  Marine and terrestrial organisms organisms  Insects  Small mammals www.jonesctr.org/.../ food_plot_study.html  Bird eggs or nestlings

4. 03 May 2004Coventry DoughertyBiol 466H Presentation Reproductive Biology  American Crows are seasonal breeders breeders Starling Breeding Cycle Dawson & Goldsmith, 1983

5. 03 May 2004Coventry DoughertyBiol 466H Presentation Purpose of Study  The mechanism regulating ovarian growth and regression is unknown growth and regression is unknown Hypothesis  Change in ovarian size is due to apoptosis is due to apoptosis  Goal: To gain an understanding of the molecular & cellular processes molecular & cellular processes regulating ovarian growth & regression regulating ovarian growth & regression

6. 03 May 2004Coventry DoughertyBiol 466H Presentation Apoptosis (Programmed Cell Death) http://www-micro.msb.le.ac.uk/3035/kalmakoff/baculo/pics/Apoptosis.gif

7. 03 May 2004Coventry DoughertyBiol 466H Presentation Methodology  Tissue processing ~ Embedding ~ Embedding ~ Sectioning ~ Sectioning  Tissue acquisition ~ CA Dept. of Fish ~ CA Dept. of Fish and Game and Game instruct.tri-c.edu/lfamian/ Ovarian%20Cycle.htm instruct.tri-c.edu/lfamian/ Ovarian%20Cycle.htm  Tissue analysis ~ Follicle state ~ Follicle state ~ ICC ~ ICC ~ TUNEL ~ TUNEL

8. 03 May 2004Coventry DoughertyBiol 466H Presentation Preliminary Data  A trend towards increasing ovarian mass March: n = 11 April: n = 11

9. 03 May 2004Coventry DoughertyBiol 466H Presentation Expected Results  Change in ovary mass  TUNEL  ICC ~ Bcl-2 ~ Bcl-2 ~ caspase-3 ~ caspase-3

10. 03 May 2004Coventry DoughertyBiol 466H Presentation Conclusion  WNV  Omnivorous  Broader Impact ~ species-specific ~ species-specific contraceptive contraceptive  Mechanism regulating ovarian growth and ovarian growth and regression regression

11. 03 May 2004Coventry DoughertyBiol 466H Presentation Acknowledgements  Howard Hughes Medical Institute  Dr. Kelly A. Young, California State University, Long Beach  Dr. A. Z. Mason, California State University, Long Beach  HHMI Program Coordinators Dr. Margaret Merryfield & Cecilia Arriaza, California State University, Long Beach Dr. Margaret Merryfield & Cecilia Arriaza, California State University, Long Beach  Lab Partners Kim Jenkins & Chantelle Moffat-Blue, California State Kim Jenkins & Chantelle Moffat-Blue, California State University, Long Beach University, Long Beach