1. DCFH-DA DCFH-DA DCFH DCF H O 2 2 2 2 Oxidative Burst

2. Fluorescent Dyes Fluorescein (FITC) 400 nm600 nm700 nm Wavelength 500 nm Excitation Emission

3. Phycoerythrin (PE) 400 nm600 nm700 nm Wavelength Excitation Emission 500 nm Fluorescent Dyes

4. Immunophenotyping CD2 CD4 CD # = cluster designation number

5. Log FITC 1000 100 10 1.1 Immunophenotyping

6. Log FITC Fluorescence (CD8).1 1 10 1001000 12 34 45% 2% 26% CD4/CD8 Quadstats 27%

7. G2G2G2G2 M G0G0G0G0 G1G1G1G1 s 0 200 400 600 8001000 G0G0G0G0 G1G1G1G1 s G2G2G2G2M DNA Analysis DNA content CountCount 2N 4N Normal Cell Cycle

8. 0 200 400 600 8001000 PI Fluorescence DNA Analysis 2N 4N

9. 0 200 400 600 8001000 PI Fluorescence DNA Analysis Aneuploid peak DNA index 1.21

10. Chromosome Analysis chromosome 1 chromosome 2 (BivariateFlow Karyotyping) (Bivariate Flow Karyotyping)

11. log Thiazole Orange.1 1000 100 10 1 RMI = 0 log Thiazole Orange.1 1000 100 10 1 R1R1R1R1 R2R2R2R2 R3R3R3R3 R4R4R4R4 RMI = 34 Reticulocyte Analysis R1R1R1R1 R2R2R2R2 R3R3R3R3 R4R4R4R4

12. Calcium Flux - Indo-1

13. 90 Degree Scatter 0 200 400 600 8001000 8 15 20 30 40 50 100 200 1000 Lymphocytes Monocytes Neutrophils Side Scatter Projection Light Scatter Gating Scale

14. Phagocytic Vacuole SOD H2O2H2O2H2O2H2O2 NADPH NADP O2O2O2O2 NADPH Oxidase OH - O2-O2-O2-O2- 