1. Cell Biology Unit One

2. Cell Measurements Micrometer (micron) –one millionth of a meter

3. Cell Measurements Nanometer (millimicron) –one billionth of a meter

4. Robert Hooke

5. Antoine van Leeuwenhoek

6. Robert Brown

7. Matthias Schleiden

8. Theodor Schwann

9. Karl Nageli

10. Rudolf Virchow

11. The Cell Theory All organisms consist of one or more cells The cell is the basic unit of life All cells arise from pre-existing cells

12. Cell Biology Foundation s Biochemistry Cytology Genetics

13. Cell Biology Foundations Cytology - the study of the structure of the cell (300+ years old) - improved microscopy has led to the discovery of cell structures and functions -led to the discipline of histology

14. Cell Biology Foundations Biochemistry -the chemistry of organisms (200 years old) -discovery that organics are produced from inorganics -discovery of enzymes

15. Cell Biology Foundations Biochemistry -discovery of metabolic pathways -discovery and use of improved methods and equipment

16. Cell Biology Foundations Genetics - the function of genes and flow of information from one generation to the next (150 years old) -Mendel’s pioneering work in the 1860’s

17. Cell Biology Foundations Genetics -identification of chromosomes and the chromosome theory of heredity -DNA the major component of chromosomes -discovery and role of genes

18. Cell Biology Foundations Genetics -Watson & Crick DNA model -cracking the genetic code -development of recombinant DNA technology, DNA sequencing, cloning, etc.

19. The Compound Light Microscope

20. The Compound Light Microscope

21. Phase-Contrast Microscopy

22. Differential Interference Contrast Microscopy

23. Fluorescence Microscopy

24. Electron Microscopes

25. Electron Microscopes

26. Transmission Electron Microscopes

27. Transmission Electron Microscopy Images

28. Scanning Electron Microscopes

29. Scanning Electron Microscopy Images

30. Electron Microscopy Preparation Tools and Techniques Ultra microtome Negative Staining

31. Electron Microscopy Preparation Tools and Techniques Freeze Fracturing Shadowing

32. Other Cell Biology Techniques Centrifugation - read Box 12A pages 322-326 - read Box 12A pages 322-326 Chromatography - read pages 164-165 - read pages 164-165 Electrophoresis - read page 176, diagram 177 - read page 176, diagram 177