1. Activity #2: Ethanol and Tobacco Toxicity in Living Animal Cells

2. Step 1 Infusion Infusion of hay, dry grass, or pond mud Opacity Check: try reading a newspaper through the culture jar to check for cloudiness

3. Step 2 - Treatments Add various substances to other culture tubes –Water (leaves one tube untreated as a control) –Alcohol, tobacco –Here we also tested mouthwash and toothpaste

4. Step 3a – Examine Tubes Visually Observe and Note Immediate Effects (Repeat again after two days) Water (control) Ethanol Tobacco Mouthwash Toothpaste Coca Cola

5. Step 3b Inspect the Organisms Examine Organisms Under the Microscope – Initially and Two Days Later

7. Step 4. Effects of Treatment Over Time As seen under a microscope, number and type of protists should change rapidly – in a matter of hours

8. Step 5 DNA Extraction Water (control) Ethanol Tobacco Mouthwash Toothpaste Coca Cola DNA

9. Water (control) DNA Mainly Salt

10. Ethanol Mainly Salt

11. Tobacco DNA Salt

12. Mouthwash DNA

13. Toothpaste DNA

14. Coca Cola DNA