1. Turfgrass Entomology Update Rutgers University Albrecht Koppenhöfer

2. Mating disruption of oriental beetleMating disruption of oriental beetle Biological and biorational control of annual bluegrass weevilBiological and biorational control of annual bluegrass weevil Steinernema scarabaei for long term white grub suppressionSteinernema scarabaei for long term white grub suppression Entomopathogenic nematode application and ecologyEntomopathogenic nematode application and ecology New chemistry for white grubs and annual bluegrass weevil managementNew chemistry for white grubs and annual bluegrass weevil management Research Projects:

3. isolated from epizootics in Japanese and oriental beetle larvae in turfgrass areas in central NJisolated from epizootics in Japanese and oriental beetle larvae in turfgrass areas in central NJ biology similar to that of other EPN speciesbiology similar to that of other EPN species appears to be rather specific to white grubsappears to be rather specific to white grubs Steinernema scarabaei for long term white grub suppression Nematode-infected grubs S. scarabaei H. bacteriophora High pathogenicity and high field efficacy against many white grub speciesHigh pathogenicity and high field efficacy against many white grub species

4. Long-term suppression of white grubs by S. scarabaei - Field experiments - 4’ x 4’ Kentucky bluegrass plots enclosed by 5” high ‘Emerald Edge’.4’ x 4’ Kentucky bluegrass plots enclosed by 5” high ‘Emerald Edge’. 160 OB larvae (late L2 & early L3; 10/ft2) released 1 wk before treatment.160 OB larvae (late L2 & early L3; 10/ft2) released 1 wk before treatment. Application with watering can (~0.5” water) in mid-September (early L3).Application with watering can (~0.5” water) in mid-September (early L3). Field irrigated as necessary to keep out of excessive drought stress.Field irrigated as necessary to keep out of excessive drought stress.

5. Annual White Grub Seasonallife-cycle Timing of sampling activities EPNEPN EPNGrubEPNGrub Appl.EPN

6. Long term suppression of white grubs by S. scarabaei Expt. 1 Treated 9/17/02 0-2.5 B Ss/ha

7. Long-term suppression of white grubs by S. scarabaei Expt. 2 Treated 9/19/03 0-0.6 B Ss/ha

8. Long-term suppression of A. orientalis in turfgrass by S. scarabaei Field experiments (1.5 m 2 microplots, 10 L3/0.1 m 2 ) Months after S. scarabaei-Application Ss rate (x 10 9 /ha) 1 (Oct) 8 (May) 13 (Oct) 20 (May) 25 (Oct) 32 (May) 37 (Oct) 0.4 – 2.5 86–10096–10062–9269–940–9463–830–64 0.1 – 0.25 50–7786–10076–7793–9533–50--- 0.06989411008---

9. Conclusions Conclusions: S. scarabaei for white grub control: 1.has outstanding potential as a curative control agent for many white grub species. 2.suppresses grub populations for 1-2 years after application. 3.is effective in a wide variety of soil types. 4.may be a good candidate for inoculative releases.