1. March 15, 2007 Update Presentation P E M PemPlant Co. Maricela Delgadillo Kay Furman Yushan Kim

2. PemPlant Co. 2 Bacteria Experiment Timepoints: 2h, 4h General steps ▪Grow up S. epi. ▪Incubate samples in bacteria-water solution for timepoints ▪Place agar gel on top ▪Incubate overnight PEM CompositionSubstrate PAH/PAA, 2.0/2.0Silane glass PAAm/PAA, 3.0/3.0Silane glass NoneGlass

3. PemPlant Co. 3 Bacteria Experiment Inconclusive results: Positive control did not yield bacterial growth Possible problems ▪Weak strain ▪Experimentalist error: ethanol? Next steps: ▪Order new strain ▪Be more careful

4. PemPlant Co. 4 Initial Validation: Methylene Blue Binds to carboxylate groups Indicates presence of PEM’s PAA surface Immerse in 1 M MB, pH 7.0 Rinse in DI H 2 O

5. PemPlant Co. 5 Methylene Blue Results Undipped Ti3.0/3.0 PAAm/PAA Results after drying Areas of stain reflects PEM presence

6. PemPlant Co. 6 Current & Future Steps This week: 2.0/2.0 PAH/PAA on Ti ▪Methylene Blue validation Mechanical MFP analysis ▪All samples Wear test Contact angle measurements for PEM presence validation Bacteria repeat, including Ti PEMs