1. Bioprocess Engineering 基因重組﹝ PCR, host selection ﹞ 或 微生物篩選 ﹝ plasmid selection ﹞ Lab. 生產工廠生產 前處理﹝ 殺菌等 ﹞生化反應器破碎細胞 分離及純化 Recombinant protein metabolites 細胞固定化﹝ 酵 素固定化 ﹞ 04.1 養菌 &bioreactor

2. ﹝一﹞前處理 1. 高溫高壓﹝ 125 ℃ ~140 ℃ ﹞ heat sterilizer, auto clave 2. 化學藥品 酒精， detergent ﹝ SDS … 等﹞ 3. 過濾 殺菌：

3. colony

4. Isolation of single colony

5. Antibiotics resistance

7. rich media defined media Determining cell mass dry weight optical density (O.D.) spectrophotometer O.D. 550 O.D. 600

11. ﹝二﹞生化反應器 1.Fermentor 發酵槽 ﹝ batch ﹞ 2.chemostat

12. Chemostat: cellular growth is usually limited by one essential nutrient, and other nutrients are in excess (P183,184)

13. Turbidostat: The cell concentration in the culture vessel is maintained constant by monitoring the optical density of the culture and controlling the feed flow pate. (P184)

14. The ideal chemostat:

15. 使用 chemostat 為 bioreactor ，其產量將遠 大於 batch 。可是為什麼工業界使用 batch 的比 率遠高於 chemostat ？ 1.Secondary products 2.Genetic instability 3.Operability and reliability 4.Market economics