1. Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo Identify dys fusion cell enhancer dys-gal4::UAS-CD8-GFPbtl-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFPdys-gal4::UAS-CD8-GFP ; dys/dys Identify dys target gene Co-activator or repressor of Dys::Tgo?

2. LP05454 Exon1234567 8910 dys 2 (1.7kb) dys1 (2.7kb) dys un (3.8kb) dys intron 2a (3.8kb) dys intron2b (3.8kb) Identify dys enhancer dys1a (1.7kb) dys1b (1.0kb) dys intron1 (1.4kb) dys1b-A (500bp)dys1b-B (500bp)

3. Enhancer for brain, hindgut and anal pad dys intron-1dys intron-2b dys intron-2a

4. dys1 fragment contains dys fusion cell enhancer dys un (S15-17) dys1 (S12-17) dys2 ( S15-17) DT LT DB Leading edge? DB DT LT S14 S17 S16 S14

5. dys1b (S12-17) S14 S16 dys1a (S14-17) S15 S14 S12

6. dys target gene: CG15252 Exon1 CG15252 con (3.2kb) 9.0 kB 5’ upstream CG15252 TCGTG ( 0.8 kb) TCGTG

7. CG15252 enhancer CG15252 con CG15252 TCGTG DT S16

8. CG15252 enhancer TCGTG-a 185 bp TCGTG-b 360 ssbp TCGTG a+b (270bp)TCGTG c+d (100bp) TCGTG-c 774 bp TCGTG-d 792 bp 818bp TCGTG to GATCCmutation:(waiting for result) ( NO?)(Yes!)

9. CG15252 enhancer: 100bp with 2 TCGTG sites DT S16 S17

10. CG13196 500bp 350bp 100bp? CG13196-500 CG13196-350 490bp

11. Ungoing..... CG15252 DT expression depends on Sal? CG15252-GFP expression in Sal mutant? Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-sal; UAS-dys Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-kni; UAS-dys Co-activator or repressor of Dys::Tgo? Compare enhancer of CG13196 and CG15252, identify potential binding sites (drifter?)