1. Fundamental Concepts in Genetic Logic Design - Principles of Control, Digital and Analog Devices Medicine 2B 徐廷儀 2009/03/11

2. Positive Feedback Loop

3. Characteristics of the Components Constitutively expressed Inducible repression

4. Positive Feedback Loop Applications Immediate expression Tremendous expression Expression maintenance

5. Negative Feedback Loop

6. Characteristics of the Components Constitutively expressed Inducible and repressible expression

7. Negative Feedback Loop Applications Moderate expression Stabilized expression Homeostasis

8. Multistable Feedback Loop

9. Characteristics of the Components Constitutively expressed Repressible expression

10. Multistable Feedback Loop Applications Fate-determining Initial condition memorizing

11. Projects Review Positive feedback loop –Jam07_St. Petersburg Negative feedback loop –Jam07_St. Petersburg –Jam08_BrownTwo Multistable feedback loop –Jam07_Brown –Jam07_St. Petersburg

12. Frequently Used Components Lac Operon –pLac, lacI, LacR, IPTG Lux Operon –pLux, luxI, LuxI, luxR, LuxR, SAM, AHL Tet Operon –pTet, tetR, TetR, aTc AraC Operon –pAraC, araC, AraC, L-arabinose Reporters –GFP, RFP, YFP… RBS Terminator

13. Strength Measurement Jam07_Brown