1. Solid Phase Peptide Synthesis (SPPS) 1963 – Reported by Bruce Merrifield 1984 – Nobel prize awarded to Merrifield

2. Polystrene solid support Linker coupling reagent Fmoc Solid Phase Peptide Synthesis – Loading AA1 onto Resin

3. Fmoc Solid Phase Peptide Synthesis of Ala-Phe-Val Ala-Phe-Val

4. Fuzeon® is a 36 Amino Acid Peptide for HIV therapy One of the most complex drugs chemically synthesized on large scale Manufactured at Roche Pharmaceuticals Boulder, Colorado

5. www.fuzeon.com Fuzeon® Interferes with HIV Entry Into T-Cells – Inhibits Fusion of Viral & Cellular Membranes Protease inhibitors Reverse transcriptase inhibitors Fusion inhibitor Fuzeon® binds to a glycopeptide on the HIV viral envelope and prevents T-cell entry Reduces HIV viral load and increases number of T-cells