1. Copyright © 2010 Pearson Education, Inc. Lectures prepared by Christine L. Case Chapter 13 Viruses, Viroids, and Prions

2. Copyright © 2010 Pearson Education, Inc. Q&A  Researchers converted skin cells into embryonic stem cells. They first inserted RNA complementary for four embryonic genes into a virus; the resulting provirus inserted the genes into the skin cells’ DNA. What virus can do this?

3. Copyright © 2010 Pearson Education, Inc. General Characteristics of Viruses 13-1Differentiate a virus from a bacterium. Learning Objective

4. Copyright © 2010 Pearson Education, Inc. General Characteristics of Viruses  Obligatory intracellular parasites  Contain DNA or RNA  Contain a protein coat  Some are enclosed by an envelope  Some viruses have spikes  Most viruses infect only specific types of cells in one host  Host range is determined by specific host attachment sites and cellular factors

5. Copyright © 2010 Pearson Education, Inc. Figure 13.1 Virus Sizes

6. Copyright © 2010 Pearson Education, Inc. Check Your Understanding How could the small size of viruses have helped researchers detect viruses before the invention of the electron microscope? 13-1

7. Copyright © 2010 Pearson Education, Inc. Viral Structure 13-2Describe the chemical and physical structure of both an enveloped and a nonenveloped virus. Learning Objective

8. Copyright © 2010 Pearson Education, Inc. Virion Structure Figure 13.2a  Nucleic acid  DNA or RNA  Capsid  Capsomeres  Envelope  Spikes

9. Copyright © 2010 Pearson Education, Inc. Figure 13.2 Morphology of a Polyhedral Virus

10. Copyright © 2010 Pearson Education, Inc. Figure 13.16a Polyhedral Viruses

11. Copyright © 2010 Pearson Education, Inc. Figure 13.3 Morphology of an Enveloped Virus

12. Copyright © 2010 Pearson Education, Inc. Figure 13.16b Enveloped Viruses

13. Copyright © 2010 Pearson Education, Inc. Figure 13.4 Morphology of a Helical Virus

14. Copyright © 2010 Pearson Education, Inc. Figure 13.5 Morphology of a Complex Virus

15. Copyright © 2010 Pearson Education, Inc. Check Your Understanding Diagram a nonenveloped polyhedral virus that has spikes. 13-2

16. Copyright © 2010 Pearson Education, Inc. 13-3Define viral species. 13-4Give an example of a family, genus, and common name for a virus. Taxonomy of Viruses Learning Objectives

17. Copyright © 2010 Pearson Education, Inc. Taxonomy of Viruses  Family names end in -viridae.  Genus names end in -virus.  Viral species: A group of viruses sharing the same genetic information and ecological niche (host). Common names are used for species.  Subspecies are designated by a number.

18. Copyright © 2010 Pearson Education, Inc. Taxonomy of Viruses  Herpesviridae  Herpesvirus  Human herpes virus HHV-1, HHV-2, HHV-3  Retroviridae  Lentivirus  Human immunodeficiency virus HIV-1, HIV-2

19. Copyright © 2010 Pearson Education, Inc. Check Your Understanding How does a virus species differ from a bacterial species? 13-3 Attach the proper endings to Papilloma- to show the family and genus that includes HPV, the cause of cervical cancer. 13-4

20. Copyright © 2010 Pearson Education, Inc. Isolation, Cultivation, and Identification 13-5Describe how bacteriophages are cultured. 13-6Describe how animal viruses are cultured. 13-7List three techniques used to identify viruses. Learning Objectives

21. Copyright © 2010 Pearson Education, Inc. Figure 13.6 Growing Viruses  Viruses must be grown in living cells  Bacteriophages form plaques on a lawn of bacteria

22. Copyright © 2010 Pearson Education, Inc. Figure 13.7 Growing Viruses  Animal viruses may be grown in living animals or in embryonated eggs

23. Copyright © 2010 Pearson Education, Inc. Figure 13.8 Growing Viruses  Animal and plant viruses may be grown in cell culture  Continuous cell lines may be maintained indefinitely

24. Copyright © 2010 Pearson Education, Inc. Virus Identification  Cytopathic effects  Serological tests  Detect antibodies against viruses in a patient  Use antibodies to identify viruses in neutralization tests, viral hemagglutination, and Western blot  Nucleic acids  RFLPs  PCR

25. Copyright © 2010 Pearson Education, Inc. Figure 13.9 Virus Identification

26. Copyright © 2010 Pearson Education, Inc. Check Your Understanding What is the plaque method? 13-5 Why are continuous cell lines of more practical use than primary cell lines for culturing viruses? 13-6 What tests could you use to identify influenza virus in a patient? 13-7

27. Copyright © 2010 Pearson Education, Inc. Viral Multiplication 13-8Describe the lytic cycle of T-even bacteriophages. 13-9Describe the lysogenic cycle of bacteriophage lambda. Learning Objectives

28. Copyright © 2010 Pearson Education, Inc. The Lytic Cycle  Attachment: Phage attaches by tail fibers to host cell  Penetration: Phage lysozyme opens cell wall; tail sheath contracts to force tail core and DNA into cell  Biosynthesis: Production of phage DNA and proteins  Maturation: Assembly of phage particles  Release: Phage lysozyme breaks cell wall

29. Copyright © 2010 Pearson Education, Inc. Figure 13.10 A Viral One-Step Growth Curve

30. Copyright © 2010 Pearson Education, Inc. Lytic Cycle of a T-Even Bacteriophage 1 2 3 Figure 13.11

31. Copyright © 2010 Pearson Education, Inc. 4 Figure 13.11 Lytic Cycle of a T-Even Bacteriophage

32. Copyright © 2010 Pearson Education, Inc. Results of Multiplication of Bacteriophages  Lytic cycle  Phage causes lysis and death of host cell  Lysogenic cycle  Prophage DNA incorporated in host DNA  Phage conversion  Specialized transduction ANIMATION Viral Replication: Temperate Bacteriophages ANIMATION Viral Replication: Virulent Bacteriophages

33. Copyright © 2010 Pearson Education, Inc. Figure 13.12 The Lysogenic Cycle

34. Copyright © 2010 Pearson Education, Inc. 2 3 4 5 6 Figure 8.28 Generalized Transduction

35. Copyright © 2010 Pearson Education, Inc. Figure 13.13 Specialized Transduction

36. Copyright © 2010 Pearson Education, Inc. 2 3 4 5 6 Specialized Transduction ANIMATION Transduction: Specialized Transduction ANIMATION Transduction: Generalized Transduction

37. Copyright © 2010 Pearson Education, Inc. Check Your Understanding How do bacteriophages get nucleotides and amino acids if they don’t have any metabolic enzymes? 13-8 Vibrio cholerae produces toxin and is capable of causing cholera only when it is lysogenic. What does this mean? 13-9

38. Copyright © 2010 Pearson Education, Inc. Viral Multiplication 13-10Compare and contrast the multiplication cycle of DNA- and RNA-containing animal viruses. Learning Objective

39. Copyright © 2010 Pearson Education, Inc. Multiplication of Animal Viruses  Attachment: Viruses attach to cell membrane  Penetration by endocytosis or fusion  Uncoating by viral or host enzymes  Biosynthesis: Production of nucleic acid and proteins  Maturation: Nucleic acid and capsid proteins assemble  Release by budding (enveloped viruses) or rupture

40. Copyright © 2010 Pearson Education, Inc. Figure 13.14a Attachment, Penetration, Uncoating  By pinocytosis

41. Copyright © 2010 Pearson Education, Inc. Figure 13.14b Attachment, Penetration, Uncoating  By fusion

42. Copyright © 2010 Pearson Education, Inc. Figure 13.20 Budding of an Enveloped Virus

43. Copyright © 2010 Pearson Education, Inc. Figure 13.20 Budding of an Enveloped Virus

44. Copyright © 2010 Pearson Education, Inc. Figure 13.15 Multiplication of DNA Virus

45. Copyright © 2010 Pearson Education, Inc. Figure 13.17a Sense Strand (+ Strand) RNA Virus

46. Copyright © 2010 Pearson Education, Inc. Figure 13.17b Antisense Strand (– Strand) RNA Virus

47. Copyright © 2010 Pearson Education, Inc. Figure 13.17c Double-Stranded RNA Virus

48. Copyright © 2010 Pearson Education, Inc. Figure 13.17 Multiplication of RNA-Containing Viruses

49. Copyright © 2010 Pearson Education, Inc. Figure 13.19 Multiplication of a Retrovirus

50. Copyright © 2010 Pearson Education, Inc. ANIMATION Viral Replication: Animal Viruses ANIMATION Viral Replication: Overview

51. Copyright © 2010 Pearson Education, Inc. Check Your Understanding Describe the principal events of attachment, entry, uncoating, biosynthesis, maturation, and release of an enveloped DNA-containing virus. 13-10

52. Copyright © 2010 Pearson Education, Inc. Viruses and Cancer 13-11Define oncogene and transformed cell. 13-12Discuss the relationship between DNA- and RNA-containing viruses and cancer. Learning Objectives

53. Copyright © 2010 Pearson Education, Inc. Cancer  Activated oncogenes transform normal cells into cancerous cells  Transformed cells have increased growth, loss of contact inhibition, tumor-specific transplant antigens, and T antigens  The genetic material of oncogenic viruses becomes integrated into the host cell's DNA

54. Copyright © 2010 Pearson Education, Inc. Oncogenic Viruses  Oncogenic DNA viruses  Adenoviridae  Herpesviridae  Poxviridae  Papovaviridae  Hepadnaviridae  Oncogenic RNA viruses  Retroviridae  Viral RNA is transcribed to DNA, which can integrate into host DNA  HTLV-1  HTLV-2

55. Copyright © 2010 Pearson Education, Inc. Check Your Understanding What is a provirus? 13-11 How can an RNA virus cause cancer if it doesn’t have DNA to insert into a cell’s genome? 13-12

56. Copyright © 2010 Pearson Education, Inc. Latent and Persistent Viral Infections 13-13Provide an example of a latent viral infection. 13-14Differentiate persistent viral infections from latent viral infections. Learning Objectives

57. Copyright © 2010 Pearson Education, Inc. Figure 13.21 Latent and Persistent Viral Infections

58. Copyright © 2010 Pearson Education, Inc.  Virus remains in asymptomatic host cell for long periods  Cold sores, shingles Latent Viral Infections Figure 13.21

59. Copyright © 2010 Pearson Education, Inc.  Disease processes occurs over a long period; generally is fatal  Subacute sclerosing panencephalitis (measles virus) Persistent Viral Infections Figure 13.21

60. Copyright © 2010 Pearson Education, Inc. Check Your Understanding Is shingles a persistent or latent infection? 13-13, 13-14

61. Copyright © 2010 Pearson Education, Inc. Prions, Viroids, and Plant Viruses 13-15Discuss how a protein can be infectious. 13-16Differentiate virus, viroid, and prion. 13-17Describe the lytic cycle for a plant virus. Learning Objectives

62. Copyright © 2010 Pearson Education, Inc. Prions  Proteinaceous Infectious particle  Inherited and transmissible by ingestion, transplant, and surgical instruments  Spongiform encephalopathies: Sheep scrapie, Creutzfeldt- Jakob disease, Gerstmann-Sträussler-Scheinker syndrome, fatal familial insomnia, mad cow disease

63. Copyright © 2010 Pearson Education, Inc. Prions  PrP C : Normal cellular prion protein, on cell surface  PrP Sc : Scrapie protein; accumulates in brain cells, forming plaques ANIMATION Prion: Diseases ANIMATION Prion: Characteristics ANIMATION Prion: Overview

64. Copyright © 2010 Pearson Education, Inc. Figure 13.22 How a Protein Can Be Infectious

65. Copyright © 2010 Pearson Education, Inc. Figure 13.23 Plant Viruses and Viroids  Plant viruses: Enter through wounds or via insects  Viroids: Infectious RNA; e.g., potato spindle tuber disease

66. Copyright © 2010 Pearson Education, Inc.

67. Check Your Understanding Contrast viroids and prions, and for each name a disease it causes. 13-15, 13-16 How do plant viruses enter host cells? 13-17

68. Copyright © 2010 Pearson Education, Inc. Virus Families That Affect Humans 13-10Compare and contrast the multiplication cycle of DNA and RNA containing animal viruses. Learning Objective

69. Copyright © 2010 Pearson Education, Inc. Table 13.2 Parvoviridae  Single-stranded DNA, nonenveloped viruses  Fifth disease  Anemia in immunocompromised patients

70. Copyright © 2010 Pearson Education, Inc. Figure 13.16a Adenoviridae  Double-stranded DNA, nonenveloped viruses  Respiratory infections in humans  Tumors in animals

71. Copyright © 2010 Pearson Education, Inc. Table 13.2 Papovaviridae  Double-stranded DNA, nonenveloped viruses  Papillomavirus  Human wart virus  Polyomavirus  Cause tumors; some cause cancer

72. Copyright © 2010 Pearson Education, Inc. Figure 13.5b Poxviridae  Double-stranded DNA, enveloped viruses  Orthopoxvirus (vaccinia and smallpox viruses)  Molluscipoxvirus  Smallpox  Molluscum contagiosum  Cowpox

73. Copyright © 2010 Pearson Education, Inc.  Double-stranded DNA, enveloped viruses  Simplexvirus (HHV-1 and HHV-2)  Varicellovirus (HHV-3)  Lymphocryptovirus (HHV-4)  Cytomegalovirus (HHV-5)  Roseolovirus (HHV-6)  HHV-7  Kaposi's sarcoma (HHV-8)  Some herpesviruses can remain latent in host cells Figure 13.16b Herpesviridae

74. Copyright © 2010 Pearson Education, Inc. Figure 25.15 Hepadnaviridae  Double-stranded DNA, enveloped viruses  Hepatitis B virus  Use reverse transcriptase

75. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, + strand, nonenveloped  Enterovirus  Poliovirus and coxsackievirus  Rhinovirus  Hepatitis A virus Table 13.2 Picornaviridae

76. Copyright © 2010 Pearson Education, Inc. Table 13.2 Caliciviridae  Single-stranded RNA, + strand, nonenveloped  Hepatitis E virus  Norovirus causes gastroenteritis

77. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, + strand, enveloped  Alphavirus  Transmitted by arthropods; includes EEE and WEE  Rubivirus (rubella virus) Table 13.2 Togaviridae

78. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, + strand, enveloped  Arboviruses can replicate in arthropods; include yellow fever, dengue, SLE, and West Nile viruses  Hepatitis C virus Clinical Focus, p. 223 Flaviviridae

79. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, + strand, enveloped  Upper respiratory infections  Coronavirus  SARS Table 13.2 Coronaviridae

80. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, – strand, one RNA strand  Vesiculovirus  Lyssavirus (rabies virus)  Cause numerous animal diseases Figure 13.18a Rhabdoviridae

81. Copyright © 2010 Pearson Education, Inc. Filoviridae  Single-stranded RNA, – strand, one RNA strand  Filovirus  Enveloped, helical viruses  Ebola and Marburg viruses Figure 23.21

82. Copyright © 2010 Pearson Education, Inc. Table 13.2 Paramyxoviridae  Single-stranded RNA, – strand, one RNA strand  Paramyxovirus  Morbillivirus  Parainfluenza  Mumps  Newcastle disease (chickens)

83. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, – strand, one RNA strand  Hepatitis D virus  Depends on coinfection with hepadnavirus Table 13.2 Deltaviridae

84. Copyright © 2010 Pearson Education, Inc.  Single-stranded RNA, – strand, multiple RNA strands  Envelope spikes can agglutinate RBCs  Influenzavirus (influenza viruses A and B)  Influenza C virus Table 13.2 Orthomyxoviridae

85. Copyright © 2010 Pearson Education, Inc. Avian Influenza Clinical Focus, p. 371

86. Copyright © 2010 Pearson Education, Inc. Bunyaviridae  Single-stranded RNA, – strand, multiple RNA strands  Bunyavirus (CE virus)  Hantavirus Table 13.2

87. Copyright © 2010 Pearson Education, Inc. Arenaviridae  Single-stranded RNA, – strand, multiple RNA strands  Helical capsids contain RNA-containing granules  Lymphocytic choriomeningitis  VEE and Lassa fever Table 13.2

88. Copyright © 2010 Pearson Education, Inc. Retroviridae  Single-stranded RNA, 2 RNA strands, produce DNA  Use reverse transcriptase to produce DNA from viral genome  Lentivirus (HIV)  Oncogenic viruses  Includes all RNA tumor viruses Figure 19.13

89. Copyright © 2010 Pearson Education, Inc. Reoviridae  Double-stranded RNA, nonenveloped  Reovirus (respiratory enteric orphan)  Rotavirus (mild respiratory infections and gastroenteritis)  Colorado tick fever Table 13.2

90. Copyright © 2010 Pearson Education, Inc. Q&A  Researchers converted skin cells into embryonic stem cells. They first inserted RNA complementary for four embryonic genes into a virus; the resulting provirus inserted the genes into the skin cells’ DNA. What virus can do this?