1. b

2. Eosinophil

4. Peripheral Blood

5. Erythrocytes = RBCs NO NUCLEUS Contain hemoglobin

6. Platelets

7. Leukocytes = WBCs Granulocytes Neutrophils = PMNs = “polys” Eosinophils = “eos” Basophils = “basos” Agranulocytes Lymphocytes = T and B cells B cells  plasma cells Monocytes  Macrophages (M Φ )

8. Granulocytes PMNEosBaso PMN

9. Neutrophils Numerous Multilobed nucleus Azurophilic & Specific granules Phagocytose bacteria

10. Basophils Rare! Lobulated nucleus often obscured by granules Dark Blue Granules Hydrolytic enzymes, heparin sulfate, histamine, SRS Function Role in hypersensitivity and anaphylaxis

11. Eosinophils Bilobed nucleus Bright pink Granules Arginine rich major basic protein, peroxidase, histaminase, arylsulfatase Function: Important in allergic rxns, parasitic infections, and phagocytosis of Ab-Ag complexes

13. Monocytes Largest WBCs in blood smear Migrate through blood to the tissues; once in tissues they differentiate into phagocytes (macrophages, osteoclasts)

15. Lymphocytes About size of RBCs Function Adaptive immune system T & B cells

16. Questions Which of the below cell types would increase in number with a bacterial infection? Viral? Parasitic?

17. Bone Marrow Smear

18. Erythropoesis

20. Proerythroblast Biggest in lineage Large central nucleus with one or two nucleoli Basophilic cytoplasm b/c ribosomes Look for Golgi ghost

21. Basophilic Erythroblast Smaller than proerythroblast Checkerboard nucleus (heterochromatic) Intense basophilia (lots of ribosomes!)

22. Proerythroblast vs Basophilic Erythroblast

23. Polychromatophilic Erythroblast Smaller than basophilic erythroblast Smaller intensely heterochromatic nucleus Purple/lilac cytoplasm mix of basophilia from ribosomes and growing eosinophilia from hemoglobin LAST MITOTIC STAGE!

24. Normoblast Smaller than polychromatophilic erythroblast Small, compact, intensely staining nucleus that is getting ready to be extruded Eosinophilic cytoplasm due to abundant hemoglobin

25. Reticulocyte Immature RBC that has polyribosomes Appear as polychrom- atophilic erythrocyte on blood smear When stained with a special (supravital) stain  Reticulocyte

26. Erythrocyte Smallest Eosinophilic due to hemoglobin NO NUCLEUS!

27. Review

28. Granulopoesis

29. Ross Fig 9-18 p. 235

30. Development of Granulocyte (focus on PMN)

31. What are Granulocytes? WBCs that contain specific granules Granule contents vary between cell types and stain differently Neutrophils (“polys” or PMNs) Eosinophils Basophils

32. Trend of Development Larger  smaller Azure granules  specific granules Round nucleus  altered shape nucleus Granulocyte Lineage: Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Granulocyte

33. Myeloblast Prominent nucleoli

34. PROmyelocyte Details Golgi ghost Can’t distinguish type of granulocyte yet Eccentric nucleus Don’t confuse with proerythroblast or basophilic erythroblasts

35. Myelocyte Last stage in which mitosis can occur Begin to see who’s a neutrophil, who’s an eosinophil (who’s a basophil) Golgi ghost

36. Neutrophilic Myelocyte vs Eosinophilic Myelocyte

37. METAmyelocyte nucleus changing shape, not spherical No more mitosis

38. Band Cell Immature granulocyte Elongated nucleus of nearly uniform width; horseshoe/U-shaped

39. Finally...

40. Myeloblast – no granules capable of mitosis Promyelocyte – azurophilic granules Mylocyte – specific granules Metamylocyte – specific granules nucleus changes shape Band cell – (PMN only) specific granules Mature Granulocyte – specific granules

41. Review

42. Compare what you see...

43. Megakaryocyte Huge Platelets form from invaginations of this Multilobular nucleus

46. Monocyte

47. Don’t Worry, Be Happy! Animations: http://www.medtraining.org/ltac/ User: library Password: cumed Click on the “Peripheral Blood” lesson