1. Endomembranes & Protein Trafficking Chapter 8 Part 1

2. Major Intracellular Compartments of the Cell

3. Eucaryotic cell Compartments

4. Simplified Road Map of Protein Traffick

5. Protein Trafficking

6. Approaches to the Study of Endomembranes

7. Autoradiography: Site & Synthesis of Secretory Proteins

8. The Secretory Pathway: Pancreatic Acinar Cell: Autoradiography showing movement of labeled secretory proteins (red color = black silver grains)

9. Use of GFP reveals movement of proteins within a living cell

11. Biochemical Analysis of Subcellular Fractions

12. Cell-Free Systems Formation of Coated Vesicles using liposomes incubated with protein components identified to be required for vesicle budding in a cell

13. Use of Genetic Mutants in the Study of Secretion: The Yeast Model

14. The Endoplasmic Reticulum (ER)

16. Polarized Structure of a Secretory Cell Mucus-secreting cells from Rat Colon

17. Overview of Protein Sorting

18. Isolation of Rough ER (Microsomes)

19. The Signal Hypothesis Experiment Incorporation of Secretory Proteins into Microsomes

20. The Signal Hypothesis Experiment Incorporation of Secretory Proteins into Microsomes

21. The Signal Hypothesis Experiment Incorporation of Secretory Proteins into Microsomes SDS-Gel Electrophoresis Analysis Translation on free ribosomesTranslation on Microsomes Secretory Protein

22. The Signal Hypothesis Continued

24. The Signal Sequence of Growth Hormone Signal Peptidase Processing Enzyme located in ER

25. Schematic Model of the synthesis of a Secretory Protein (or a lysosomal enzyme) on an Membrane-Bound ribosome of the RER.

26. Role of GTP Hydrolysis in SRP and SRP Receptor Function

27. Only for General Knowledge

28. Schematic Model for the Synthesis of an Integral Membrane Protein

29. Only for General Knowledge Maintenance of Membrane Asymmetry

30. Only for General Knowledge Modifying the Lipid Composition of Membranes

31. Only for General Knowledge Synthesis of the core portion of an N-linked oligosaccharide in the rough ER

32. Quality Control: Ensuring that Misfolded proteins do not proceed forward

33. Model of the Mammalian Unfolded Response UPR