1. Solid-state Fermentation
by using DFM(Direct-fed Microorganism)
for Feed & Feed-additives

2. Application of Solid-state Fermentation
Foods: Functional-foods and Food-ingredients
Livestock: Probiotics
Agricultural Area: Bio-fertilizer
Pharmaceuticals: Functional Bio-active Ingredients
장수농기센터와 유용미생물과의 만남
가축질병진단실 담당자로서 고민
축산용 생균제 알게되엇고 생균제는 보조단미와 동물용의약품으로 분류
유용미생물 기본개념

3. Microbial Fermentation
Method
Liquid-state Fermentation: Alcohol Production
Solid-state Fermentation: Fermented Food
Controlling Parameters
Chemical Factors: Nutrients, Growth Factors, etc
Physical Factors: Temp., pH, Oxygen, etc
Biological Factors:

4. Solid-state Fermentation
Advantage
Less contamination by low water content
Oxygen-supply, CO2-removal and temp-control by aeration
Disadvantage
Difficulty in Mixing: Low in homogeneousness
Difficulty in temp-control by low conductivity
Difficulty in On-line monitoring
Experience-dependent

5. DFM(Probiotics) in Livestock
Direct-fed Microorganism
Probiotics
Definition: Microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

6. Design of Solid-state Fermentor
Exhaust Line
& Evaporation
Door for
Raw Materials-Supply
& Inspection
Liquid-supply
Spray Nozzle
Paddle-type
Mixing Device
Recovery for
Final Products
Aeration Holes: Temp-controlled
Air-Supply through chamber
Forced air-supply by Blower
With Temp-controlled Air

7. Overall Fermentation Processes
Preparatory
Stage
Main
Fermentation
Manufacturing
Stage
Raw Materials:
Feedstuff or
Fermentation media
Control
Temp, Aeration & Mixing
Microbial Growth
Water Content
Control:
Water content
Temp & Mixing
DFM(Probiotics)
Packaging
Two-stage
Fermentation
1st Stage:
Aerobic Process
2nd Stage:
Anaerobic Process
TMF
Water Content
Functioanl
Ingredients

8. Solid-state Fermentation Process
Preparatory Stage
Media as Raw Materials: Feedstuff
DMF(Probiotics): High-density Microbial Cells
Functional Ingredients: Growth factors
1st Stage: Aerobic Fermentation
Microbial Growth (Proliferation) & Enzyme Production
Providing Aerobic Condition:
Air supply through aeration holes at the bottom of fermentor
Continuous agitation (mixing) for aeration and mixing
2nd Stage: Anaerobic Fermentation
Stabilization of grown-microorganisms
Producing Bio-active Microbial Metabolites & Flavours
Simultaneous providing anaerobic condition:
Air supply through top of fermentor according to temp-control

9. DFM(Probiotics) for Fermentation
Effective Microorganisms
used for Solid-state Fermentation
Lactobacillus species
Bacillus species
Aspergillus species
Yeast (Saccharomyces) species

10. TMF (Total Mixed Fermentation)
TMR (Total Mixed Ration)
Feedstuffs for Dairy Cow
Simple Mixing, None-Fermentation
TMF: Advanced TMR
Fermented Feedstuffs
Fermented DMF(Probiotics)
Composition:
Live Probiotics: Effective Live-microorganisms
Microbial components: Bio-active Microbial Products & Metabolites
Microbial Enzymes: Various Enzymes

11. Function of TMF in Livestock
Normalization of Microflora
Protection of inflammation and diarrhea
Prevention of various diseases
Enhancement in immunity and feed efficiency
Minimizing undigested materials
Providing various bio-active compounds
TMF
In Vivo
Minimizing fiscal smell and water content
Effective for Waste-treatment
Providing Well-being Environment
Reduction in insect plague
In Vitro

12. Equipments for DFM(Probiotics)
Refrigerator for
Stock and media
Clean bench
For aseptic work
Shaking Incubator for seed culture
Lab-scale &
Pilot-scale Fermentor
Incubator for microbial growth

13. Equipments for TMF Solid-state Fermentor Supply-Device
For Raw Materials
Screw Conveyor
Dumping
Transfer Devices
For TMF(Product)
Screw Conveyor
Solid-state Fermentor
Liquid-supply Devices
For DFM & Liquids
FRP Tank & Feeding Pump
Packing Device
For Manufacturing
Packing & Wraping

14. Solid-state Fermentor (I)
Aeration Unit
Ring blower with Line heater
Dual way for aeration: Top & Bottom of Fermentor
Controlled by PLC (Temp & Process)
Agitation (mixing) Unit
Paddle-type Impeller
Mixing & Holding
Controlled by PLC (Temp, Aeration & Process)
Others
Liquid-supply Unit:
Special Screw Unit: Pushing-out final product

15. Solid-state Fermentor (II)
Control Unit
PLC control with Programmed Software
Interlocked with Aeration-Temp-Agitation-Process Time
Data-storage and Ethernet Communication
User-friendly Interface
Consultation
Support by Highly Experienced & Educated Expertise
Experiences in Manufacturing & Process Control
Consultation in Design, Process & Manufacturing, Products
A/S & On-site Support