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Circadian Susceptibility to Pyrethroids in Female Drosophila melanogaster By David Chin Mentor: Dr. Jadwiga Giebultowicz
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Relevance for Research How fruit flies metabolize toxins may be: Different between males and females Related to how humans metabolize toxins via circadian rhythms These links may open up new inquiries in pharmaceuticals, horticulture, and global economics
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Circadian Rhythms Internal clocks that control when organisms rest and remain active I.e. Jet lag… … … Controlled by the Period and Tim genes http://edfredned.com/images/portfolio/Full/jetlag.gif
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Circadian Clocks (cont.) http://www.ou.edu/wanglab/Circadianclock1/Slide1.jpg
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Circadian Rhythms (cont.) Physiologically, they influence: Hormone secretion Oxygen consumption Metabolism Other functions of the body
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Point of Interest Circadian rhythms may influence the rate of metabolism of many substances, including toxins The pyrethroid, deltamethrin, is administered to the female flies to investigate how they metabolize toxins in relation to the presence and absence of the circadian rhythm
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Pyrethroids Synthetic insecticides derived from the flowers of pyrethrums or several plants from the genus Chrysanthemum http://upload.wikimedia.org/wikipedia/com mons/f/fa/Chrysanthemums.jpg
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Deltamethrin Deltamethrin is a pyrethroid insecticide that damages the nervous system, paralyzing the organisms after cuticular penetration or oral uptake of the neurotoxin http://upload.wikimedia.org/wikipedia/commons/3/3f/Deltamethrin-3D-balls-2.png C 22 H 19 Br 2 NO 3
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Deltamethrin (cont.) Modifies the membrane function proteins and keeps the voltage-dependent sodium channels continuously open
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Flies Being Tested CS Flies = Wild type flies with Period & Tim genes Per-null Flies = Mutant flies that lack the Period gene expressions Csp Flies = Backcrossed flies that express the Period & Tim genes and are closely related to the Per-null flies
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Experiments Dose Response Two Hour Interval Test GST Assay Glutathione S-Transferases take part in metabolizing toxins
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Mass Ratio Data Average mass of female = 1.409 mg Average mass of male = 0.8103 mg
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Dose Response Data
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Two Hour Interval Test Data
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Csp and Per-Null Testing Data
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GST Assay Data
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Future Investigations I will continue working on investigating direct toxin applications and GST assays throughout the 2007-2008 school year at Oregon State University
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Acknowledgements Howard Hughes Medical Institute URISC Dr. Kevin Ahern Dr. Jadwiga Giebultowicz Everyone in the lab
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