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Katy McHenry & Dr. Fredrick Stormshak Department of Animal Sciences Oregon State University
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Bovine Estrous Cycle = 21 days One egg matures Released as an oocyte Granulosa cells of the empty follicle turn into the corpus luteum Angiogenesis Endocrine Gland
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Stimulation of milk letdown Smooth muscle contraction in uterus during childbirth Roll in maternal instinct and building relationships?
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Bovine luteal oxytocin acts locally to stimulate the proliferation and migration of endothelial cells to form the vascular system of the developing corpus luteum.
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1. Block oxytocin receptors Corpus Luteum can not form 2. Tumor angiogenesis and growth Progesterone
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1. Isolate the endothelial cells 2. Measure the binding of oxytocin to endothelial cell oxytocin receptors
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Remove corpus luteum eight days post-estrus Remove corpus luteum eight days post-estrus Check cows for estrus using vasectomized bull Check cows for estrus using vasectomized bull
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Dissociate corpus luteum using collagenase and DNAse Tosyl-activated magnetic beads Couple with lectin BS-1 Add dissociated tissue and separate endothelial cells
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[ H]-Oxytocin & 200 fold excess unlabeled Oxytocin [ H]-Oxytocin [ H]-Oxytocin 3 3
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Results
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Results
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New Theory Pericyte Cells Stem Cells Smooth Muscle
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Results
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Summary Oxytocin showed slight binding to receptors on endothelial cells in the corpus luteum. Hypothesis was not strongly supported Large amount of oxytocin binding to receptors on non-endothelial luteal cells Possibly pericyte cells?
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Dr. Fred Stormshak Oregon State University, Department of Animal Sciences Oregon State University, Honors College Dr. Kevin Ahern The Howard Hughes Medical Institute
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