1. Microbiology Unknown Lab BIO 187

2. TSI agar Triple Sugar Iron Agar
0.1% dextrose
1.0% sucrose
1.0% lactose
TSI agar Triple Sugar Iron Agar
A B C D E
(a) Red/red (no sugar fermentation)
(b) Control
(c) Red/yellow (Glucose fermented but lactose and sucrose not fermented)
(d) Yellow/yellow (Glucose fermented. Lactose and/or sucrose fermented)
(e) Red/yellow with H2S

3. Penol Red broths
A B C D E
A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E (alkaline reaction)

4. O-F tests Control E. coli Pseudomonas Alcaligenes
Fermenter Oxidizer Nonsaccharolytic

5. Motility Test

6. Decarboxylation
uninoculated
Positive Negative

7. Gelatin Hydrolysis

8. Simmons Citrate A: Positive…Enterobacter B: Negative…E. coli
A B C
A: Positive…Enterobacter
B: Negative…E. coli
C: Control-uninoculated

9. Phenylalanine Deaminase Test
neg pos

10. Urea agar
pos uninoculated neg

11. Urea Hydrolysis
Positive uninoculated Negative

12. Methyl Red Test
A B
A: Positive (E. coli) B: Negative (Enterobacter aerogenes)

13. Voges Proskauer Test A B
A: Negative (E. coli) B: Positive (Enterobacter aerogenes)

14. Indole Test
Negative Positive

15. Catalase Test
Positive Negative

16. GRAM STAIN

17. Urea Hydrolysis
Positive uninoculated Negative

18. General Microbiology (BIOL 187) Laboratory Report (Unknown Identification)
Page 1: Cover page
Include your name, the unknown number and you final Genus and species identification
Page 2: Test listing
I want a (+) / (-) listing of each test in a vertical, easily scanned format
Page 3: Discussion section
Detail, in complete sentences, why your results justify your identification and rule out other possibilities.
Page 4: About My Microbe section
Research your microbe and give me a several paragraphs about your microbe.
(Don't copy word-for-word from your textbook---This will be frowned upon!)
Page 5: Dichotomous key
Create a neat dichotomous key, which results in the correct separation of all 8 possible unknowns. This key should be legible and sized to occupy a complete sheet of paper (nothing microscopic is acceptable here)
Page 6:Lab handout where you recorded your initial results
Include the handout from lab where your recorded your data as you read the test reactions.
Grading:
20 pts. Correct Identification
10 pts. Discussion section
7 pts. "About my microbe section"
8 pts. Dichotomous key
5 pts. Neatness, accuracy and completeness of report
50 points total