1. Panzea Allele Frequency Display Tutorial www.panzea.org With the Panzea Allele Frequency Geographic Display tool, you can display pie graphs that show the geographic distribution of allele frequencies for a SNP, SSR or isozyme marker The display is based upon Google Maps. Hence, you can use their standard controls to change between map, satellite or hybrid view or to move or resize the map Upon moving or resizing the map, the grid upon which the allele frequencies are based is redrawn, and new pie graphs are calculated and displayed

2. www.panzea.org Tasks demonstrated: 1)Choose the marker of interest and display initial map 2)Change to “hybrid” (combined map & satellite) view 3)Choose a single species or subspecies to display 4)Change the zoom level 5)Obtain underlying information for a pie graph 6)Simplify pie graphs by pooling low frequency alleles 7)Display pie graphs for a single allele 8)Reposition the map Panzea Allele Frequency Display Tutorial

3. www.panzea.org 1) Choose marker of interest and display initial map

4. www.panzea.org 1) Choose marker of interest and display initial map We will use an SSR marker as an example, so here we will only change the Marker Type, and leave the other options in their default settings

5. www.panzea.org 1) Choose marker of interest and display initial map For example

6. www.panzea.org 2) Change to ‘hybrid’ (combined map & satellite) view

7. www.panzea.org 3) Choose a single species or subspecies to display spp. mays will display maize landraces only (since inbred lines do not have geographical coordinates) Then click the “Filter” button (hidden under this drop down list in this view)

8. www.panzea.org 4) Change the zoom level Click here to zoom out

9. www.panzea.org 5) Obtain underlying information for a pie graph Click on a pie chart for details

10. www.panzea.org 5) Obtain underlying information for a pie graph Details for that pie chart then appear here. Use the scroll bar at the right to see them all

11. www.panzea.org 6) Simplify pie graphs by pooling low freq. alleles

12. www.panzea.org 6) Simplify pie graphs by pooling low freq. alleles

13. www.panzea.org 7) Display pie graphs for a single allele Choose allele 92 (for example), and then click the “Filter” button (hidden under this drop down list in this view)

14. www.panzea.org 7) Display pie graphs for a single allele

15. www.panzea.org 8) Reposition the map Use these arrow buttons to move the map, or click on the map and drag (hand tool)

16. www.panzea.org 8) Reposition the map