1. Relationship of Gene’s Type and Introns Chi K. To

2. Eukaryotes contain multiple introns per gene. Throughout eukaryotic evolution, the length of introns had been changed by either insertion, deletion or both. How big of these lengths had been change? Do they just happen in specific type of genes? What are those types?

3. Method: Method: Use the Browser to get introns in the whole genome. Do pairwise alignment between human and dog genomic sequences with the reconstructed sequences of their common ancestor. Only select introns have gaps that are greater than 100bp.

4. Insertion (gaps on ancestral sequences), deletion (gaps on human/dog sequences). Get genes’ IDs Upload data to the http://gostat.wehi.edu.au/ - help to calculate the Hypergeometric distribution for the data http://gostat.wehi.edu.au/ Gene ontology classification of introns.

5. Result: GO ID Descriptionp-val GO:0030695 Mol. Function: GTPase regulator activity [Modulates the rate of GTP hydrolysis by a GTPase] 1.89E-05 GO:0005886 Location: plasma membrane 1.01E-04 GO: 0005887 Integral to plasma membrane 2.3E-04 GO: 0006607 Biological process: NLS-bearing substrate-nucleus 5.42E-04 Insertion in Human

6. Go ID Descriptionp-val GO:0030695 Mol. Function: GTPase regulator activity 5.49E-06 GO: 0005083 Mol. Function: small GTPase regulatory/interacting protein activity 3.65E-04 GO: 0005085 Mol. Function: guanyl-cucleotide exchange factor activity 6.89E-04 GO: 0008092 Mol. Function: cytoskeletal protein binding 1.96E-03 Deletion in Human

7. Go ID Descriptionp-val GO:0030695 Mol. Function: small GTPase regulatory/interacting protein activity 5.35E-04 GO:0006607 Biological process: NLS-bearing substrate-nucleus 5.35E-04 GO:0030036 Bio. Process: Actin cytoskeleton organization and biogenesis 2.67E-03 GO:0005587 Cellular component: collagen type IV 2.67E-03 Insertion in Dog

8. Go ID Descriptionp-val GO:0030036 Actin cytoskeleton organization and biogenesis 2.53E-04 GO:0030029 Bio. Process: Actin filament-based process 2.75E-04 GO:0005083 Mol. Function: small GTPase regulatory/interacting protein activity 4.06E-04 GO:0005886 Location: plasma membrane 4.8E-04 Deletion in Dog

9. * In human: Genes have the molecular function related to GTPase regulator activity or locate in plasma membrane tend to change the lengths of introns within the genes during the evolutionary process. * In human: Genes have the molecular function related to GTPase regulator activity or locate in plasma membrane tend to change the lengths of introns within the genes during the evolutionary process. * In Dog: Genes have the biological process related to Actin cytoskeleton organization and biogenesis and the mol. munction related to small GTPase regulatory/interacting protein activity tend to change the lengths of introns within the genes during the evolution.

10. A big deletion of a intron in gene NM_018283 of Dog

11. Special thanks to: Special thanks to: 1/ Baertsch, Robert – pairwise alignment data 1/ Baertsch, Robert – pairwise alignment data 2/ http://gostat.wehi.edu.au/ - for the G.O. statistics 2/ http://gostat.wehi.edu.au/ - for the G.O. statisticshttp://gostat.wehi.edu.au/ 3/ UCSC genome Browser 3/ UCSC genome Browser