1. A Metabolomic Approach to the Identification of Cancer Biomarkers
Clive Baveghems
Bioanalytical Chemistry
Spring 2011

2. Outline What is Metabolomics?
Metabolomic screening for the identification of cancer biomarkers
Metabolomic screening for the identification of prostate cancer biomarkers

3. Introduction to Metabolomics
Sawyer, J. Surg. Oncol. 2010; 21831: 2

4. Yearly increase in Metabolomics publications
Note: 2010 total ~ 750
Ong, Trends in Analytical Chemistry 2010; 29(3):270

5. Factors influencing the Metabolome
Sawyer, J. Surg. Oncol. 2010; 21831: 2

6. Outline What is Metabolomics?√
Metabolomic screening for the identification of cancer biomarkers
Metabolomic screening for the identification of prostate cancer biomarkers

7. Biomarker Identification
Sawyer, J. Surg. Oncol. 2010; 21831: 4

8. Cancer biomarkers identified so far
Sawyer, J. Surg. Oncol. 2010; 21831: 5

9. Key Metabolites implicated so far
Sawyer, J. Surg. Oncol. 2010; 21831: 6

10. Outline What is Metabolomics?√
Metabolomic screening for the identification of cancer biomarkers√
Metabolomic screening for the identification of prostate cancer biomarkers

11. Prostate Cancer From 2003-2007 median age for diagnosis was 67
The age-adjusted incidence rate was 156.9/100, 000 men per year
The median age at death was 80
The age-adjusted death rate was 24.7/100, 000 men per year

12. Results from LC and GC-MS
Chinnaiyan, Nature 2009; 457(12): 911

13. Sarcosine identified as a biomarker for prostate cancer
Chinnaiyan, Nature 2009; 457(12): 913

14. Is sarcosine a biomarker for prostate cancer?
GC-MS data
Jung, Journal of Urology 2011; 185: 708

15. Summary
Metabolomics has emerged as a fast and efficient method for the identification of a small set of candidate biomarkers from a large eclectic data set
LC/MS, GC/MS, and NMR are important metabolomic technologies
Future studies will further explain sarcosine’s role in Prostate Cancer

16. References
Metabolomics and Surgical Oncology: Potential Role for Small Molecule Biomarkers Sawyer, J. Surg. Oncol. 2010; 21831: 1-9
Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression Chinnaiyan, Nature 2009; 457(12):
Sarcosine in Prostate Cancer Tissue is Not a Differential Metabolite for Prostate Cancer Progressiveness and Biochemical Progression Jung, Journal of Urology 2011; 185:
Experiment-originated variations, and multi-peak and muti-origination phenomena in derivatization-based GC-MS metabolomics Ong, Trends in Analytical Chemistry 2010; 29(3):