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Do They Eat Petroleum Hydrocarbons? Bioremediation of Toluene by Oil-Water Separator Bacteria LISA L. LOFTIS and JAMES E. CHAMPINE. Biology Department, Southeast Missouri State University.
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Site History Former Gasoline Station Soil and Groundwater Contamination Why Remediation System was chosen
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Site Map
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Soil Vapor Extraction Trailer
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Hypothesis Contaminated groundwater represents a natural enrichment for petroleum hydrocarbon degrading bacteria
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Approach Use toluene as a surrogate for petroleum related hydrocarbons that may be degraded in the aquifer Test groundwater biofilm and pure cultures from the site for toluene degradation activity
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Site Materials Before and After groundwater samples Oil/Water Separator Biofilm (Jar and Vial) Collection of Bacterial Isolates
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Gas Chromatograph
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Chromatography Conditions Carrier Gas DB1 30 m capillary column 200ºC oven temperature Flame Ionization Detector
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Typical chromatogram
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Serum Bottle Cultures 20 mL of sterile strength 0.1x TSB Teflon Rubber Stoppers Aluminum Crimps Amended with 10 L of Toluene Aerobic
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Serum Bottle Cultures
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Before Groundwater Experiment
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After Groundwater Experiment
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Isolations Before Beige (from After groundwater) ECP 6 ECP 7
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Toluene Degraders ECP 6 and ECP 7
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Conclusions Intrinsic Bioremediation did occur at the site Site cleaned up in half of the estimated time Bacteria with toluene degradation activity were isolated
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Acknowledgments Southeast Missouri State University Undergraduate Research Program Heather DuShell Dr. Bjorn Olesen and the Department of Chemistry Vicki Howell, Biology Dept. Secretary
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