1. Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “  ” key to view slides…

2. Lab 1: Eukaryotes and Microscopy Know the parts of the microscope…

3. Lab 1: Eukaryotes and Microscopy Paramecium caudatum

4. Lab 1: Eukaryotes and Microscopy Euglena gracilis

5. Lab 1: Eukaryotes and Microscopy Amoeba proteus

6. Lab 1: Eukaryotes and Microscopy Fungus – Saccharomyces cerevisiae

7. Lab 1: Eukaryotes and Microscopy Cyanobacteria ~ Nostoc

8. ~Bacteria slides~ Micrococcus luteus

9. ~Bacteria Slides~ Bacillus subtilis

10. ~Bacteria Slides~ Bacillus anthracis

11. ~Bacteria Slides~ Rhodospirillum rubrum Sorry not in focus…

12. ~Bacteria Slides~ See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining… [ Adobe Acrobat of Gram and Endospore ]Adobe Acrobat of Gram and Endospore Some Endospore Staining ~

13. Streak Plate: Know colony characteristics (color, elevation, margin, etc.)

14. Spread Plate vs. Streak Plate:

15. Streak Plate:

16. Spread Plate: Mixed – Bacillus subtilis & Serratia marcescens

17. Practical I Unknown Tests: ~ Simple Stain ~ Gram Stain ~ Endospore Stain ~ Acid-Fast Stain (Demo) ~ Capsule Stain (Demo) ~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep) ~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)

18. Gas Pak Anaerobic vs. Aerobic:

19. Dilution Problems:

21. Bacterial Growth Curve: Make sure you know how to: Use a spectrophotometer to measure absorbance. Produce a log graph of the calculations. Calculate generation time from a growth curve.