1. BIPN 148 Lecture 12

2. Biochemical Basis of LTP

3. Interaction Between AMPAR, Stargazin, and PSD-95 Stargazin interacts with AMPA receptors in an intracellular compartment in the cell and promotes their delivery to the cell surface. The carboxyl terminus of stargazin binds specifically to the anchor protein PSD-95 and mediates recruitment of the stargazin-AMPA receptor complex to postsynaptic sites.

4. Loss of AMPA current in stargazer mutant mice (recordings from cerebellar granule neurons)

5. Stargazin enhances glutamate response

6. Stargazin restores AMPA currents in mutants (Stargazin-deltaC restores surface expression, but not synaptic targeting)

7. PSD95 and Stargazin have distinct effects on synaptic responses and surface AMPA receptors

8. Stargazin-deltaC inhibits AMPA responses

9. Stargazin increases AMPA channel openings

10. Stargazin increases surface GluR1 expression

11. GluR1-C tail expression inhibits LTP

12. Effect of GluR1 S887A (C tail) on LTP

13. Role of GluR1 trafficking in fear conditioning: Physiology of GluR1 transfected neurons in Amygdala

14. Expression of GluR1-C inhibits plasticityin Amygdala

15. Expression of GluuR1-C inhibits fear conditioning